caa a22 4500 467985081 CHVBK 20180323112529.0 cr unu---uuuuu 170328e19900801xx s 000 0 eng 10.1007/BF00206742 doi (NATIONALLICENCE)springer-10.1007/BF00206742 High-resolution dynamic and morphological G-bandings (GBG and GTG): a comparative study [Elektronische Daten] [Nicole Lemieux, Régen Drouin, Claude-Lise Richer] Summary: A high-resolution replication banding technique, dynamic GBG banding (G-bands after 5′-bromodeoxyuridine [BrdUrd] and Giemsa), showed that, at a resolution of 850 bands/genome, GBG banding and GTG banding (G-bands after trypsin and Giemsa) produce almost identical patterns. RBG band (R-bands after BrdUrd and Giemsa) and RHG band (R-bands after heat denaturation and Giemsa) patterns were previously shown to be only 75%-85% coincident; thus GTG banding more accurately reflects replication patterns than does RHG banding. BrdUrd synchronization uses high concentrations of BrdUrd both to substitute early replicating DNA and to arrest cells before the late bands replicate. Release from the block is via a low thymidine concentration. The banding is revealed by the fluorochrome-photolysis-Giemsa (FPG) technique and produces the GBG banding that includes concomitant staining of constitutive heterochromatin. As opposed to other replication G-banding procedures, BrdUrd synchronization and GBG banding produces a reproducible replication band pattern. The discordance between homologs after GBG banding is similar to that after GTG banding and no lateral asymmetry of the constitutive heterochromatin has been observed. Also, BrdUrd synchronization neither significantly depresses the mitotic index, nor induces chromosome breaks. Thus, GBG banding seems as clinically useful as GTG banding and provides important information regarding replication time. Springer-Verlag, 1990 Lemieux Nicole Département d'Anatomie, Faculté de Médecine, Université de Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada aut Drouin Régen Département d'Anatomie, Faculté de Médecine, Université de Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada aut Richer Claude-Lise Département d'Anatomie, Faculté de Médecine, Université de Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada aut Human Genetics Springer-Verlag 85/3(1990-08-01), 261-266 0340-6717 85:3<261 1990 85 439 https://doi.org/10.1007/BF00206742 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00206742 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lemieux Nicole Département d'Anatomie, Faculté de Médecine, Université de Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada aut NATIONALLICENCE 700 1- Drouin Régen Département d'Anatomie, Faculté de Médecine, Université de Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada aut NATIONALLICENCE 700 1- Richer Claude-Lise Département d'Anatomie, Faculté de Médecine, Université de Montréal, C.P. 6128, Succ. A, H3C 3J7, Montréal, Québec, Canada aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/3(1990-08-01), 261-266 0340-6717 85:3<261 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer