caa a22 4500 467985235 CHVBK 20180323112530.0 cr unu---uuuuu 170328e19900801xx s 000 0 eng 10.1007/BF00206754 doi (NATIONALLICENCE)springer-10.1007/BF00206754 DNA sequence analysis of the KM19 locus linked to cystic fibrosis [Elektronische Daten] Design of new oligonucleotides to remove non-specific PCR products [R. Anwar, K. Murray, P. Hedge, J. Smith, A. Markham] Summary: The PstI polymorphism detected by probe KM19 is a highly informative marker in linkage disequilibrium with the cystic fibrosis locus and has been used extensively for prenatal diagnosis. The currently available primers used for polymerase chain reaction- (PCR-) based analysis of this locus have been shown to produce spurious amplification products. In this report, we describe the sequence of the KM19 locus and the major contaminating PCR product. We have used this information to design a more specific amplification procedure for analysis of the KM19 locus. Springer-Verlag, 1990 Anwar R. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut Murray K. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut Hedge P. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut Smith J. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut Markham A. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut Human Genetics Springer-Verlag 85/3(1990-08-01), 319-323 0340-6717 85:3<319 1990 85 439 https://doi.org/10.1007/BF00206754 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00206754 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Anwar R. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut NATIONALLICENCE 700 1- Murray K. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut NATIONALLICENCE 700 1- Hedge P. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut NATIONALLICENCE 700 1- Smith J. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut NATIONALLICENCE 700 1- Markham A. ICI Diagnostics, Gadbrook Park, Rudheath, CW9 7RA, Northwich, Cheshire, UK aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/3(1990-08-01), 319-323 0340-6717 85:3<319 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer