caa a22 4500 467985731 CHVBK 20180323112531.0 cr unu---uuuuu 170328e19901001xx s 000 0 eng 10.1007/BF00194226 doi (NATIONALLICENCE)springer-10.1007/BF00194226 Locus assignment of human α-globin structural mutants by selective enzymatic amplification of α1 and α2-globin cDNAs [Elektronische Daten] [Avgi Mamalaki, Margrit Horanyi, Judith Szelenyi, Nicholas Moschonas] Summary: We have used the powerful methodology of DNA enzymatic amplification in order to assign human α-globin structural mutants to one of the two highly homologous α-globin genes. Selectively amplified α1 and α2-globin cDNAs were dot-blotted and further hybridized to synthetic oligonucleotides encompassing either the normal or the mutated sequences. The generated signals corresponded specifically to one of the two α-globin genes. Using this approach the α-globin structural mutants J-Buda and G-Pest were found to be encoded by the α2 and the α1-globin genes, respectively. Furthermore, the exact nucleotide changes were determined. We propose this technique to serve as a simple and definitive method for assigning α-globin structural mutants. Springer-Verlag, 1990 Mamalaki Avgi Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, GR-711 10, Heraklion, Greece aut Horanyi Margrit National Institute of Hematology and Blood Transfusion, Budapest, Hungary aut Szelenyi Judith National Institute of Hematology and Blood Transfusion, Budapest, Hungary aut Moschonas Nicholas Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, GR-711 10, Heraklion, Greece aut Human Genetics Springer-Verlag 85/5(1990-10-01), 509-512 0340-6717 85:5<509 1990 85 439 https://doi.org/10.1007/BF00194226 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00194226 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mamalaki Avgi Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, GR-711 10, Heraklion, Greece aut NATIONALLICENCE 700 1- Horanyi Margrit National Institute of Hematology and Blood Transfusion, Budapest, Hungary aut NATIONALLICENCE 700 1- Szelenyi Judith National Institute of Hematology and Blood Transfusion, Budapest, Hungary aut NATIONALLICENCE 700 1- Moschonas Nicholas Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, GR-711 10, Heraklion, Greece aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/5(1990-10-01), 509-512 0340-6717 85:5<509 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer