caa a22 4500 467985774 CHVBK 20180323112531.0 cr unu---uuuuu 170328e19901001xx s 000 0 eng 10.1007/BF00194222 doi (NATIONALLICENCE)springer-10.1007/BF00194222 Deletion of specific sequences or modification of centromeric chromatin are responsible for Y chromosome centromere inactivation [Elektronische Daten] [Paola Maraschio, Orsetta Zuffardi, Angela Caiulo, Eleonora Dainotti, Mauro Piantanida, Horacio Rivera, Rossella Tupler] Summary: Stable dicentric chromosomes behave as monocentrics because one of the centromeres is inactive. The cause of centromere inactivation is unknown; changes in centromere chromatin conformation and loss of centromeric DNA elements have been proposed as possible mechanisms. We studied the phenomenon of inactivation in two Y centromeres, having as a control genetically identical active Y centromeres. The two cases have the following karyotypes: 45,X/46,X,i(Y)(q12) and 46,XY/ 47,XY,+t(X;Y)(p22.3;p11.3). The analysis of the behaviour of the active and inactive Y chromosome centromeres after Da-Dapi staining, CREST immunofluorescence, and in situ hybridization with centromeric probes leads us to conclude that, in the case of the isochromosome, a true deletion of centromeric chromatin is responsible for its stability, whereas in the second case, stability of the dicentric (X;Y) is the result of centromere chromatin modification. Springer-Verlag, 1990 Maraschio Paola Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Zuffardi Orsetta Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Caiulo Angela Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Dainotti Eleonora Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Piantanida Mauro Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Rivera Horacio Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Tupler Rossella Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut Human Genetics Springer-Verlag 85/5(1990-10-01), 491-494 0340-6717 85:5<491 1990 85 439 https://doi.org/10.1007/BF00194222 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00194222 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maraschio Paola Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Zuffardi Orsetta Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Caiulo Angela Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Dainotti Eleonora Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Piantanida Mauro Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Rivera Horacio Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 700 1- Tupler Rossella Biologia Generale e Genetica Medica, Universitá di Pavia, C.P. 217, I-27100, Pavia, Italy aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/5(1990-10-01), 491-494 0340-6717 85:5<491 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer