caa a22 4500 467985847 CHVBK 20180323112531.0 cr unu---uuuuu 170328e19901001xx s 000 0 eng 10.1007/BF00194219 doi (NATIONALLICENCE)springer-10.1007/BF00194219 The gene for 17beta-hydroxysteroid dehydrogenase maps to human chromosome 17, bands q12-q21, and shows an RFLP with Sca I [Elektronische Daten] [Robert Winqvist, Hellevi Peltoketo, Veli Isomaa, Karl-Heinz Grzeschik, Arto Mannermaa, Reijo Vihko] Summary: The gene encoding human 17beta-hydroxy-steroid dehydrogenase (17-HSD; EC 1.1.1.62) is assigned to chromosome 17 by Southern blotting analyses of panels of human x rodent somatic cell hybrids and independently to 17q12-q21 using chromosomal in situ hybridization. A search for physical linkage between 17-HSD and the proto-oncogenes, THRA1 and ERBB2 (both reported to be located in this region of chromosome 17) was performed by pulsed-field gel electrophoresis (PFGE) using several rare-cutting restriction endonucleases. Because all three genes hybridized to DNA fragments of different lengths it seems unlikely that the gene for 17-HSD is located very close to THRA1 and ERBB2. Further evidence for this assumption was obtained from the absence of any coamplification of the 17-HSD gene in 9 breast tumors with amplification of the ERBB2 gene. Analyses of Southern blots of ScaI-digested DNAs from unrelated individuals from Northern Finland revealed a relatively infrequent diallelic restriction fragment length polymorphism, the allele frequencies of which were 0.04 (A1) and 0.96 (A2). Springer-Verlag, 1990 Winqvist Robert Department of Clinical Genetics, Oulu University Central Hospital, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut Peltoketo Hellevi Biocenter and Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut Isomaa Veli Biocenter and Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut Grzeschik Karl-Heinz Institut für Humangenetik der Universität, Bahnhofstrasse 7a, D-3550, Marburg, Federal Republic of Germany aut Mannermaa Arto Department of Clinical Genetics, Oulu University Central Hospital, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut Vihko Reijo Biocenter and Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut Human Genetics Springer-Verlag 85/5(1990-10-01), 473-476 0340-6717 85:5<473 1990 85 439 https://doi.org/10.1007/BF00194219 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00194219 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Winqvist Robert Department of Clinical Genetics, Oulu University Central Hospital, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut NATIONALLICENCE 700 1- Peltoketo Hellevi Biocenter and Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut NATIONALLICENCE 700 1- Isomaa Veli Biocenter and Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut NATIONALLICENCE 700 1- Grzeschik Karl-Heinz Institut für Humangenetik der Universität, Bahnhofstrasse 7a, D-3550, Marburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Mannermaa Arto Department of Clinical Genetics, Oulu University Central Hospital, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut NATIONALLICENCE 700 1- Vihko Reijo Biocenter and Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, SF-90220, Oulu, Finland aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/5(1990-10-01), 473-476 0340-6717 85:5<473 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer