caa a22 4500 467985898 CHVBK 20180323112531.0 cr unu---uuuuu 170328e19901001xx s 000 0 eng 10.1007/BF00194236 doi (NATIONALLICENCE)springer-10.1007/BF00194236 In situ chromosome preparation technique for simultaneous cytogenetic and immunocytochemical studies on cell cultures of solid tumors [Elektronische Daten] [Wolfram Henn, Jutta Lehr, Gerhard Unteregger, Ayhan Ermis, Klaus Zang] Summary: Immunophenotyping of cultured cancer cells requires intact antigenic structures; these are mostly destroyed by conventional chromosome preparation techniques. Thus, the simultaneous cytogenetic and immunocytochemical characterization of solid tumor cells appears unfeasible. Here, we describe a novel method that allows in situ chromosome preparation from monolayer cultures of solid tumor cells without affecting their immunological features. Using this technique, it is possible to achieve detailed cytogenetic data including chromosome banding together with the demonstration of cytoplasmic and nuclear antigens within the same tumor cell. Springer-Verlag, 1990 Henn Wolfram Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut Lehr Jutta Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut Unteregger Gerhard Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut Ermis Ayhan Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut Zang Klaus Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut Human Genetics Springer-Verlag 85/5(1990-10-01), 551-554 0340-6717 85:5<551 1990 85 439 https://doi.org/10.1007/BF00194236 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00194236 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henn Wolfram Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut NATIONALLICENCE 700 1- Lehr Jutta Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut NATIONALLICENCE 700 1- Unteregger Gerhard Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut NATIONALLICENCE 700 1- Ermis Ayhan Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut NATIONALLICENCE 700 1- Zang Klaus Institut für Humangenetik der Universität, D-6650, Homburg/Saar, Federal Republic of Germany aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/5(1990-10-01), 551-554 0340-6717 85:5<551 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer