caa a22 4500 46798607X CHVBK 20180323112532.0 cr unu---uuuuu 170328e19900701xx s 000 0 eng 10.1007/BF00193187 doi (NATIONALLICENCE)springer-10.1007/BF00193187 Identification of sequences of chromosome 7 that are expressed in sweat gland epithelial cells [Elektronische Daten] [Julie Burns, Georg Melmer, Johanna Rommens, John Riordan, Manuel Buchwald] Summary: This paper describes an approach that can be used to identify specifically expressed coding sequences in defined regions of genomic DNA. We developed this method to identify expressed sequences from chromosome 7 located at or near the cystic fibrosis (CF) locus. Radioactively labelled single-stranded cDNAs derived from sweat gland epithelial cells and from fibroblasts were used to screen a genomic library constructed from flow-sorted chromosomes. Differential screening of phage lifts with these two probes yielded 36 different DNA segments. By using somatic cell hybrids containing different portions of chromosome 7, four of the clones were mapped to the 7q31 region in which the CF locus is located. These four clones and two others that gave strong differential epithelial signals but that were not within 7q31 were studied further. Restriction fragment length polymorphisms (RFLPs) were identified for two of the DNA segments within 7q31 and used for linkage analysis using a panel of CF families. One DNA segment was assigned to a location centromeric to the met locus. The other marker did not show recombination with CF but was subsequently excluded from the CF region by physical mapping. Three of the six DNA segments were found to hybridize to various RNAs using the Northern technique and therefore contain portions of genes. One of the clones showed strong differential expression when epithelial tissues were compared to fibroblasts and may represent an epithelium-specific gene. Springer-Verlag, 1990 Burns Julie Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut Melmer Georg Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut Rommens Johanna Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut Riordan John Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada aut Buchwald Manuel Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut Human Genetics Springer-Verlag 85/2(1990-07-01), 151-156 0340-6717 85:2<151 1990 85 439 https://doi.org/10.1007/BF00193187 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00193187 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burns Julie Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Melmer Georg Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Rommens Johanna Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Riordan John Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Buchwald Manuel Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 85/2(1990-07-01), 151-156 0340-6717 85:2<151 1990 85 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer