caa a22 4500 467986169 CHVBK 20180323112532.0 cr unu---uuuuu 170328e19901101xx s 000 0 eng 10.1007/BF00205173 doi (NATIONALLICENCE)springer-10.1007/BF00205173 A new marker at DXS 115 useful for carrier detection in hemophilia A [Elektronische Daten] [Manfred Wehnert, Winnie Schröder, Falko Herrmann] Summary: In this brief communication we report a new intergenic polymorphism at DXS115 as a marker for detection of heterozygotes in families at risk for hemophilia A. Total genomic DNA was isolated from white blood cells, double digested by KpnI and XbaI and hybridized with EcoRI/SstI fragment of the genomic probe p482.6. The incidence of the polymorphic 5.1-kb fragment was estimated as 0.069 in a German population. A technical advantage of using the XbaI/KpnI RFLP is that both the intragenic XbaI-RFLP in intron 22 of factor VIII gene and the new intergenic RFLP can be evaluated at the same time. Springer-Verlag, 1990 Wehnert Manfred Institut für Medizinische Genetik, Ernst-Moritz-Arndt-Universität, Fleischmannstrasse 42/44, O-2200, Greifswald, Germany aut Schröder Winnie Institut für Medizinische Genetik, Ernst-Moritz-Arndt-Universität, Fleischmannstrasse 42/44, O-2200, Greifswald, Germany aut Herrmann Falko Institut für Medizinische Genetik, Ernst-Moritz-Arndt-Universität, Fleischmannstrasse 42/44, O-2200, Greifswald, Germany aut Human Genetics Springer-Verlag 86/1(1990-11-01), 59-60 0340-6717 86:1<59 1990 86 439 https://doi.org/10.1007/BF00205173 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00205173 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wehnert Manfred Institut für Medizinische Genetik, Ernst-Moritz-Arndt-Universität, Fleischmannstrasse 42/44, O-2200, Greifswald, Germany aut NATIONALLICENCE 700 1- Schröder Winnie Institut für Medizinische Genetik, Ernst-Moritz-Arndt-Universität, Fleischmannstrasse 42/44, O-2200, Greifswald, Germany aut NATIONALLICENCE 700 1- Herrmann Falko Institut für Medizinische Genetik, Ernst-Moritz-Arndt-Universität, Fleischmannstrasse 42/44, O-2200, Greifswald, Germany aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 86/1(1990-11-01), 59-60 0340-6717 86:1<59 1990 86 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer