caa a22 4500 467986231 CHVBK 20180323112532.0 cr unu---uuuuu 170328e19901101xx s 000 0 eng 10.1007/BF00205165 doi (NATIONALLICENCE)springer-10.1007/BF00205165 R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1) [Elektronische Daten] [Ei-ichi Takahashi, Tada-aki Hori, Peter O'Connell, Mark Leppert, Ray White] Summary: A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidinesynchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11-q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes. Springer-Verlag, 1990 Takahashi Ei-ichi Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, 260, Chiba, Japan aut Hori Tada-aki Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, 260, Chiba, Japan aut O'Connell Peter Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132, Salt Lake City, UT, USA aut Leppert Mark Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132, Salt Lake City, UT, USA aut White Ray Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132, Salt Lake City, UT, USA aut Human Genetics Springer-Verlag 86/1(1990-11-01), 14-16 0340-6717 86:1<14 1990 86 439 https://doi.org/10.1007/BF00205165 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00205165 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Ei-ichi Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, 260, Chiba, Japan aut NATIONALLICENCE 700 1- Hori Tada-aki Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, 260, Chiba, Japan aut NATIONALLICENCE 700 1- O'Connell Peter Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Leppert Mark Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- White Ray Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132, Salt Lake City, UT, USA aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 86/1(1990-11-01), 14-16 0340-6717 86:1<14 1990 86 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer