caa a22 4500 467986436 CHVBK 20180323112533.0 cr unu---uuuuu 170328e19901201xx s 000 0 eng 10.1007/BF00197697 doi (NATIONALLICENCE)springer-10.1007/BF00197697 Partial complementation of the Fanconi anemia defect upon transfection by heterologous DNA [Elektronische Daten] Phenotypic dissociation of chromosomal and cellular hypersensitivity to DNA cross-linking agents [C. Diatloff-Zito, F. Rosselli, J. Heddle, E. Moustacchi] Summary: Transfectants obtained by mouse DNA-mediated gene transfer in Fanconi anemia (FA) primary fibroblasts from the genetic complementation groups A and B were examined for the frequencies of chromosomal aberrations and cytotoxicity following treatments by cross-linking agents. Cells from group A (FA 150), which is the most sensitive to such agents, are partially corrected for both the chromosomal and cellular hypersensitivity to 8-methoxypsoralen photoaddition. In contrast, after treatment with mitomycin C (MMC), only the chromosomal sensitivity is re-established to a near normal level. The opposite is true for FA group B cells (FA 145), i.e. cell survival to MMC is partially corrected, whereas the frequency of MMC-induced chromosomal aberration remains close to that of the untransfected cells. The partial phenotypic correction of the two end points examined is interpreted as indicating either a gene dosage effect or the necessity of introducing more than one gene type in order to achieve complete recovery of a normal phenotype. The phenotypic dissociation between the clastogenic and cellular hypersensitivity to crosslinking agents may offer the opportunity of isolating separately the responsible gene(s) by conventional rescue techniques. Springer-Verlag, 1990 Diatloff-Zito C. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut Rosselli F. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut Heddle J. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut Moustacchi E. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut Human Genetics Springer-Verlag 86/2(1990-12-01), 151-161 0340-6717 86:2<151 1990 86 439 https://doi.org/10.1007/BF00197697 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197697 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Diatloff-Zito C. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Rosselli F. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Heddle J. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Moustacchi E. CNRS URA 1292, Institut Curie, Biologie, 26, Rue d'Ulm, F-75231, Paris Cedex 05, France aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 86/2(1990-12-01), 151-161 0340-6717 86:2<151 1990 86 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer