caa a22 4500 467986460 CHVBK 20180323112533.0 cr unu---uuuuu 170328e19901201xx s 000 0 eng 10.1007/BF00197692 doi (NATIONALLICENCE)springer-10.1007/BF00197692 The 60 and 63 kDa proteolytic peptides of the red cell membrane band-3 protein: their prevalence in human and non-human primates [Elektronische Daten] [Marcos Palatnik, Maria da Silva Simões, Zoraide Salomão Alves, Nanci Maior Laranjeira] Summary: Three phenotypes based on the polymorphism of band-3 protein from human red cells are described. Limited proteolysis of intact red cells from most individuals (homozygotes) yields a peptide of 60kDa, but in some cases (heterozygotes), there is also a 63-kDa peptide, and rarely only the single peptide of 63kDa is found. This is the first description of the 63-kDa homozygote. The interpretation that the three phenotypes are controlled by two alleles of a single autosomal locus, with no dominance, is supported by population and family studies. The frequencies of the allele, which we designate as p63, is 0.041±0.0068 in Caucasoids and 0.125±0.0121 in Negroids. The electrophoretic profiles and molecular weights of the peptides obtained with several commercial proteases from Streptomyces griseus are similar to those obtained with chymotrypsin. Whereas band-3 protein in two New-World monkeys (Saimiri and Cebus) resisted pronase attack, an Old-World monkey (Macaca mulatta) was monomorphic for a 63-kDa fragment, and in an ape (Pan troglodytes), a doublet of 62 kDa and 64 kDa was found. Band-3 protein polymorphism appears to be a good marker for genetic differentiation in human populations. Springer-Verlag, 1990 Palatnik Marcos Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut da Silva Simões Maria Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut Salomão Alves Zoraide Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut Maior Laranjeira Nanci Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut Human Genetics Springer-Verlag 86/2(1990-12-01), 126-130 0340-6717 86:2<126 1990 86 439 https://doi.org/10.1007/BF00197692 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00197692 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palatnik Marcos Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- da Silva Simões Maria Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Salomão Alves Zoraide Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Maior Laranjeira Nanci Laboratório de Pesquisa, Serviço de Hemoterapia, Hospital Universitário "Clementino Fraga Filho”, 3∘ andar, Avenida Brg. Trompowski, s/n, 21941, Ilha do Fundão, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 86/2(1990-12-01), 126-130 0340-6717 86:2<126 1990 86 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer