caa a22 4500 467987874 CHVBK 20180323112537.0 cr unu---uuuuu 170328e19901201xx s 000 0 eng 10.1007/BF02539109 doi (NATIONALLICENCE)springer-10.1007/BF02539109 Comparison of allergenic potency of five batches of Alternaria alternata for preparation of reference standard [Elektronische Daten] [Hari Vijay, Maureen Burton, David Hughes, David Copeland, N. Young] Summary: Five batches of 34-016 isolate were grown separately on synthetic revised tobacco medium for 28 days. Extracts of the mycelia were prepared and their biochemical and immunological properties were examined. The extracts had similar isoelectric focussing (IEF) patterns. In crossed-radioimmuno-electrophoresis (CRIE) using human atopic sera, each showed two dominant and two to three minor allergens. In direct radioallergosorbent (RAST) and RAST inhibition tests with human atopic sera and passive cutaneous anaphylaxis (PCA) tests with mouse IgE, all samples were of similar potency. In IgE immunoblots of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, each extract showed strongly reactive bands at 16, 18, 30, 33 and 59-100 kDa MW. These results indicate that a reference preparation ofA. alternata suitable for standardization purposes can be obtained from any batch of 34-016 isolate. Kluwer Academic Publishers, 1990 allergen nationallicence Alternaria nationallicence standard nationallicence Vijay Hari Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, K1A 0L2, Ottawa, Canada aut Burton Maureen Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, K1A 0L2, Ottawa, Canada aut Hughes David Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, K1A 0L2, Ottawa, Canada aut Copeland David 165 Charlotte Street, K1N 8K6, Ottawa, Canada aut Young N. Division of Biological Sciences, National Research Council of Canada, K1A 0R6, Ottawa, Canada aut Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 159-164 0393-5965 6:2<159 1990 6 10453 https://doi.org/10.1007/BF02539109 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02539109 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vijay Hari Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, K1A 0L2, Ottawa, Canada aut NATIONALLICENCE 700 1- Burton Maureen Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, K1A 0L2, Ottawa, Canada aut NATIONALLICENCE 700 1- Hughes David Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, K1A 0L2, Ottawa, Canada aut NATIONALLICENCE 700 1- Copeland David 165 Charlotte Street, K1N 8K6, Ottawa, Canada aut NATIONALLICENCE 700 1- Young N. Division of Biological Sciences, National Research Council of Canada, K1A 0R6, Ottawa, Canada aut NATIONALLICENCE 773 0- Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 159-164 0393-5965 6:2<159 1990 6 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer