caa a22 4500 467987882 CHVBK 20180323112537.0 cr unu---uuuuu 170328e19901201xx s 000 0 eng 10.1007/BF02539114 doi (NATIONALLICENCE)springer-10.1007/BF02539114 Immunochemical assay of airborne cat allergen: value in assessing variability of allergen shedding and evaluating human bronchial response [Elektronische Daten] [J. Lewis, Patricia Wentz, Marc Swanson, Charles Reed] Summary: Twelve cats were selected from the animal care facility to determine their individual rates of allergen shedding. Cats were placed in a lucite chamber with an air sampler attached. Radioallergosorbent (RAST) inhibition type and monoclonal two-site radioimmunosurveys (RIAs) were used to express air concentrations in allergy units (AU) orFel d 1 units), respectively. Inter-cat variability ranged from a factor of 6 (inFel d 1 units) to a factor of 100 (in AU). Production of individual cats over time varied by a factor of 10-20 (AU orFel d 1). Three of the highest shedders were placed in wire cages in a room-sized chamber with closed ventilation. Four cat-sensitive asthmatics were individually exposed to the cats in the chamber and had spiromatic and minute ventilation measurements performed while personal air sampling was performed. Allergen concentration in the chamber was higher than in homes we have measured. 11% (Fel d 1) or 17% (AU) of the allergen concentration was associated with respirable particles (<9 μm). The dose of allergen required to elicit a 20% drop in FEV1 from baseline was calculated from the measured air concentration, patients' ventilation, particle sizing data and estimated lung deposition. This dose was substantially less than that required by breathing nebulized cat extract for 3 of the 4 subjects. Kluwer Academic Publishers, 1990 allergen nationallicence cat nationallicence immunology nationallicence Lewis J. Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A. aut Wentz Patricia Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A. aut Swanson Marc Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A. aut Reed Charles Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A. aut Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 193-196 0393-5965 6:2<193 1990 6 10453 https://doi.org/10.1007/BF02539114 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02539114 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lewis J. Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A aut NATIONALLICENCE 700 1- Wentz Patricia Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A aut NATIONALLICENCE 700 1- Swanson Marc Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A aut NATIONALLICENCE 700 1- Reed Charles Mayo Clinic Scottsdale, 13400 East Shea blvd, 85259, Scottsdale, Arizona, U.S.A aut NATIONALLICENCE 773 0- Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 193-196 0393-5965 6:2<193 1990 6 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer