caa a22 4500 467987912 CHVBK 20180323112537.0 cr unu---uuuuu 170328e19901201xx s 000 0 eng 10.1007/BF02539112 doi (NATIONALLICENCE)springer-10.1007/BF02539112 Allergic reactions to basidiospores: identification of allergens [Elektronische Daten] [Samuel Lehrer, W. Horner] Summary: Recent studies have demonstrated significant skin and RAST reactivity to spore extracts of multiple basidiomycete (BS) species in some atopic subjects. The association of RAST reactivity to different basidiomycete species suggested the presence of shared allergenic determinants. This was confirmed by RAST inhibition studies with spore extracts from different basidiomycete species. Preliminary investigations suggest, however, that basidiospore allergens do not share extensive cross-reactivity with members of the Fungi Imperfecti. Immunoprint analysis of proteins fromCalvatia cyathiformis, separated by isoelectrofocusing, demonstrated a number of protein bands that bound IgE from RAST positive sera. IEF/immunoprinting revealed three allergen(s)/groups or bands (Bd) with respective pI of 3.6-4.6, 6.6 and 9.3. Only Bds 3.6-4.6 were stable at 37°C or at acid pH. Bd 9.3, the most labile, reacted with 63% of the patients' sera tested. Immunoprint inhibition studies of 6 BS species suggest that Bd 9.3 fromCalvatia cyathiformis is a common allergen in other basidiomycetes. Purified fractions ofCal c Bd 9.3 were obtained through preparative isoelectrofocusing, gel filtration, and hydrophobic interaction chromatography. These studies demonstrate that basidiospores are important fungal aeroallergens that share allergenic determinants. Further efforts are directed at isolation and characterization of major shared basidiospore allergens. Kluwer Academic Publishers, 1990 allergen nationallicence basidiospores nationallicence Lehrer Samuel Allergy and Clinical Immunology Section, Tulale University School of Medicine, 1700 Perdido Street, 70112, New Orleans, LA, U.S.A. aut Horner W. Allergy and Clinical Immunology Section, Tulale University School of Medicine, 1700 Perdido Street, 70112, New Orleans, LA, U.S.A. aut Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 181-186 0393-5965 6:2<181 1990 6 10453 https://doi.org/10.1007/BF02539112 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02539112 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lehrer Samuel Allergy and Clinical Immunology Section, Tulale University School of Medicine, 1700 Perdido Street, 70112, New Orleans, LA, U.S.A aut NATIONALLICENCE 700 1- Horner W. Allergy and Clinical Immunology Section, Tulale University School of Medicine, 1700 Perdido Street, 70112, New Orleans, LA, U.S.A aut NATIONALLICENCE 773 0- Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 181-186 0393-5965 6:2<181 1990 6 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer