caa a22 4500 467988005 CHVBK 20180323112537.0 cr unu---uuuuu 170328e19901201xx s 000 0 eng 10.1007/BF02539117 doi (NATIONALLICENCE)springer-10.1007/BF02539117 Molecular cloning of grass pollen allergens: progress and perspectives [Elektronische Daten] [Shyam Mohapatra, Robert Hill, Alec Sehon] Summary: In this report the advances made in recent years as a result of application of recombinant DNA and allied technologies in relation to isolation, molecular characterization and synthesis of principal allergens—with particular emphasis on Kentucky Bluegrass (KBG,Poa pratensis) pollen allergens—has been reviewed. Screening of a cDNA library complementary to KBG pollen mRNA led to isolation of several cDNA clones. Hybridization studies using the labelled cDNAs as probes suggested the existence of at least three major classes of clones. The nucleotide sequences of several cDNAs encoding a major group of allergenic proteins were determined. Moreover, one cDNA clone, KBG7.2 was inserted in an expression plasmid to achieve high-level expression of the corresponding peptide. The recombinant peptide was shown to be comparable to its natural counterpart with regard to its ability to bind to human IgE and IgG antibodies of allergic patients, and to elicit passive cutaneous anaphylaxis (PCA) in rats. Furthermore, KBG 7.2 encoded peptide was shown to possess at least two IgE binding epitopes. About four T cell epitopes were predicted on this peptide. The results of these studies suggest that the recombinant allergens and/or allergenic peptides may be of diagnostic and therapeutic value. Kluwer Academic Publishers, 1990 allergens nationallicence grass nationallicence pollen nationallicence Mohapatra Shyam Department of Immunology, University of Manitoba, Winnipeg, Canada aut Hill Robert MRC Group for Allergy Research, University of Manitoba, Winnipeg, Canada aut Sehon Alec Department of Immunology, University of Manitoba, Winnipeg, Canada aut Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 205-211 0393-5965 6:2<205 1990 6 10453 https://doi.org/10.1007/BF02539117 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02539117 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohapatra Shyam Department of Immunology, University of Manitoba, Winnipeg, Canada aut NATIONALLICENCE 700 1- Hill Robert MRC Group for Allergy Research, University of Manitoba, Winnipeg, Canada aut NATIONALLICENCE 700 1- Sehon Alec Department of Immunology, University of Manitoba, Winnipeg, Canada aut NATIONALLICENCE 773 0- Aerobiologia Kluwer Academic Publishers 6/2(1990-12-01), 205-211 0393-5965 6:2<205 1990 6 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer