caa a22 4500 467995397 CHVBK 20180323112555.0 cr unu---uuuuu 170328e19900701xx s 000 0 eng 10.1007/BF01244764 doi (NATIONALLICENCE)springer-10.1007/BF01244764 HPLC post column derivatization of aromatic amines using N -methyl-9-chloroacridinium triflate [Elektronische Daten] [Myungsoo Kim, James Stewart] An HPLC post column chemical derivatization procedure based on the interaction between an acridinium triflate and amines to form highly colored derivatives on-line is described for the determination of aromatic amines. Benzocaine and butesin, local anesthetic agents that contain the aromatic amine group, were used as model compounds. Reversed-phase HPLC conditions were developed for both the separation of analytes and the reaction between analytes and the acridinium triflate in the system. Three-dimensional knitted teflon shape coils and the internal diameter and length of the coils were important parameters in reducing band broadening and background noise.N-Methyl-9-chloroacridinium triflate was shown to be applicable to the determination of primary aromatic amines, selected secondary aromatic amines, hydrazides, and hydrazines. Application of the on-line chemical derivatization procedure to the analysis of pharmaceutical dosage forms containing procainamide (primary aromatic amine), isoniazid (hydrazide), and hydralazine (hydrazine) is also described. Springer-Verlag, 1990 post-column derivatization nationallicence acridinium triflate salts nationallicence aromatic amines nationallicence knitted teflon reaction coils nationallicence Kim Myungsoo Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The University of Georgia, 30602, Athens, GA, USA aut Stewart James Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The University of Georgia, 30602, Athens, GA, USA aut Microchimica Acta Springer-Verlag 102/4-6(1990-07-01), 221-232 0026-3672 102:4-6<221 1990 102 604 https://doi.org/10.1007/BF01244764 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01244764 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Myungsoo Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Stewart James Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 773 0- Microchimica Acta Springer-Verlag 102/4-6(1990-07-01), 221-232 0026-3672 102:4-6<221 1990 102 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer