caa a22 4500 467995591 CHVBK 20180323112556.0 cr unu---uuuuu 170328e19900101xx s 000 0 eng 10.1007/BF01244284 doi (NATIONALLICENCE)springer-10.1007/BF01244284 Kinetic study of Pt-cytostatics reactions by HPLC [Elektronische Daten] [Eva Brandšteterová, František Kiss, Stanislav Miertuš, Ján Garaj] HPLC with DAD (diode array detector) was applied as rapid method for the analytical control of the purity and stability of carboplatin and oxocarboplatin as effective cytostatics of 2nd generation. The stability of carboplatin has been measured in some infusion media (sodium chloride, glucose, sodium citrate) and compared to the cisplatin (cytostatic of 1st generation) stability. In addition ligand-exchange reactions between cisplatin and carboplatin have been studied together with the influence of temperature, pH and the concentrations of reactants. A method was developed for the analysis of clinical samples using solid-phase extraction as a preseparation and preconcentration step. The detection limits were 5 μg/ml serum for cisplatin, 0.16 μg/ml for carboplatin and 0.23 μg/ml for oxo-carboplatin. Springer-Verlag, 1990 cisplatin nationallicence carboplatin nationallicence HPLC determination nationallicence solid-phase extraction nationallicence serum analysis nationallicence DAD detection nationallicence Brandšteterová Eva Department of Analytical Chemistry, Slovak Technical University, Radlinského 9, CS-812 37, Bratislava, Czechoslovakia aut Kiss František Research Institute, Lachema, CS-621 33, Brno, Czechoslovakia aut Miertuš Stanislav Department of Analytical Chemistry, Slovak Technical University, Radlinského 9, CS-812 37, Bratislava, Czechoslovakia aut Garaj Ján Department of Analytical Chemistry, Slovak Technical University, Radlinského 9, CS-812 37, Bratislava, Czechoslovakia aut Microchimica Acta Springer-Verlag 102/1-3(1990-01-01), 11-19 0026-3672 102:1-3<11 1990 102 604 https://doi.org/10.1007/BF01244284 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01244284 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brandšteterová Eva Department of Analytical Chemistry, Slovak Technical University, Radlinského 9, CS-812 37, Bratislava, Czechoslovakia aut NATIONALLICENCE 700 1- Kiss František Research Institute, Lachema, CS-621 33, Brno, Czechoslovakia aut NATIONALLICENCE 700 1- Miertuš Stanislav Department of Analytical Chemistry, Slovak Technical University, Radlinského 9, CS-812 37, Bratislava, Czechoslovakia aut NATIONALLICENCE 700 1- Garaj Ján Department of Analytical Chemistry, Slovak Technical University, Radlinského 9, CS-812 37, Bratislava, Czechoslovakia aut NATIONALLICENCE 773 0- Microchimica Acta Springer-Verlag 102/1-3(1990-01-01), 11-19 0026-3672 102:1-3<11 1990 102 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer