caa a22 4500 469026146 CHVBK 20180323132730.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF01970169 doi (NATIONALLICENCE)springer-10.1007/BF01970169 Drug transport across the blood — brain barrier [Elektronische Daten] II. Experimental techniques to study drug transport [J. Van Bree, A. De Boer, M. Danhof, D. Breimer] This is part II of a review on the transport of drugs across the blood-brain barrier. In this part, the emphasis is on the various experimental techniques that can be used to characterize the blood — brain barrier transport of drugs. Generally speaking, three approaches can be distinguished:in vitro techniques using isolated brain capillaries, cerebrovascular endothelial cells in primary culture or endothelium-derived cell lines;in vivo techniques (both single-passage and multi-passage techniques) andin situ perfusion techniques. Each of these techniques has specific advantages and disadvantages associated with it. Therefore, in many instances, a combination of different approaches is needed to study the fundamental aspects of drug transport across the blood-brain barrier. Royal Dutch Association for Advancement of Pharmacy, 1992 Biological transport nationallicence Blood — brain barrier nationallicence Cell membrane permeability nationallicence Cerebrospinal fluid nationallicence Methods nationallicence Research nationallicence Van Bree J. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut De Boer A. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut Danhof M. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut Breimer D. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut Pharmaceutisch Weekblad Kluwer Academic Publishers 14/6(1992-12-01), 338-348 0031-6911 14:6<338 1992 14 11096 https://doi.org/10.1007/BF01970169 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01970169 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Bree J. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut NATIONALLICENCE 700 1- De Boer A. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut NATIONALLICENCE 700 1- Danhof M. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut NATIONALLICENCE 700 1- Breimer D. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands aut NATIONALLICENCE 773 0- Pharmaceutisch Weekblad Kluwer Academic Publishers 14/6(1992-12-01), 338-348 0031-6911 14:6<338 1992 14 11096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469026146 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer