caa a22 4500 469026316 CHVBK 20180323132731.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01962691 doi (NATIONALLICENCE)springer-10.1007/BF01962691 Direct measurement of probenecid and its glucuronide conjugate by means of high pressure liquid chromatography in plasma and urine of humans [Elektronische Daten] [T. Vree, E. Kolmer] Probenecid with its phase-I metabolites, and phase-II glucuronide conjugate can be analysed by a gradient high pressure liquid chromatographic method. Probenecid glucuronide in plasma with pH 7.4 is not stable and declines to 10% of the original value within 6 h (t1/2≈1 h). Probenecid glucuronide is stable in urine with pH 5.0, moderately unstable at pH 6.0 (t1/2≈10 h), and unstable at pH 8.0 (t1/2≈0.5 h). Probenecid glucuronide is stable in water and 0.01 mol/l phosphoric acid in the autosampler of the high pressure liquid chromatograph. The decrease in concentration in water is 5.5% during 9 h and 0% in diluted acid. Probenecid glucuronide and the phase-I metabolites were not detectable in plasma. The main compound in fresh urine is the phase-II conjugate probenecid glucuronide (62% of a 500 mg dose); the phase-I metabolites are present and only a trace of probenecid is present. The percentage of the dose of the phase-I metabolites varies between 5 and 10, while hardly any probenecid is excreted unchanged (0.33%). Royal Dutch Association for Advancement of Pharmacy, 1992 Chromatography, high pressure liquid nationallicence Metabolites nationallicence Pharmacokinetics nationallicence Probenecid nationallicence Vree T. Department of Clinical Pharmacy and Institute of Anaesthesiology, University Hospital Nijmegen Sint Radboud, P.O. Box 9101, 6500, HB Nijmegen, the Netherlands aut Kolmer E. Department of Clinical Pharmacy, University Hospital Nijmegen Sint Radboud, the Netherlands aut Pharmaceutisch Weekblad Kluwer Academic Publishers 14/3(1992-06-01), 83-87 0031-6911 14:3<83 1992 14 11096 https://doi.org/10.1007/BF01962691 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01962691 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vree T. Department of Clinical Pharmacy and Institute of Anaesthesiology, University Hospital Nijmegen Sint Radboud, P.O. Box 9101, 6500, HB Nijmegen, the Netherlands aut NATIONALLICENCE 700 1- Kolmer E. Department of Clinical Pharmacy, University Hospital Nijmegen Sint Radboud, the Netherlands aut NATIONALLICENCE 773 0- Pharmaceutisch Weekblad Kluwer Academic Publishers 14/3(1992-06-01), 83-87 0031-6911 14:3<83 1992 14 11096 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer