caa a22 4500 469027215 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00232423 doi (NATIONALLICENCE)springer-10.1007/BF00232423 Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana [Elektronische Daten] [B. Kost, I. Potrykus, G. Neuhaus] Summary: We present protocols for the regeneration of fertile plants from immature zygotic embryos of Arabidopsis thaliana ecotype "Zürich” either directly by multiple shoot formation from the apical region or indirectly by shoot induction from embryo derived calli. The regeneration efficiency by "multiple shooting” depended on the developmental stage of the cultured embryos and ranged from 15% for early heart shaped to 90% for early torpedo shaped and further developed embryos. 85% callus induction was achieved from embryos in the early torpedo shaped or a later stage of development. The efficiency of shoot induction from embryo derived calli varied between 25% and 75% in different experiments. Springer-Verlag, 1992 Kost B. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland aut Potrykus I. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland aut Neuhaus G. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland aut Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 50-54 0721-7714 12:1<50 1992 12 299 https://doi.org/10.1007/BF00232423 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232423 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kost B. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Potrykus I. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Neuhaus G. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 50-54 0721-7714 12:1<50 1992 12 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer