caa a22 4500 469027223 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00232421 doi (NATIONALLICENCE)springer-10.1007/BF00232421 Micropropagation of black pepper ( Piper nigrum Linn.) through shoot tip cultures [Elektronische Daten] [V. Philip, Dominic Joseph, G. Triggs, N. Dickinson] Summary: The morphogenetic potential of shoot tip explants of black pepper (Piper nigrum) was investigated and an effective multiple-shoot propagation method is described. Various combinations of media, growth regulators and sterilization treatments were compared. Problems with establishment in tissue culture sometimes occurred, probably caused by endogenous pathogens associated with tissue exudates. The best establishment and proliferation of shoot tip explants was obtained on MS medium containing 1.5 mg l−1 BAP alone; subsequent growth and development of lateral branches was best on media containing 1.5 mg l−1 BAP plus 3.0 mg l−1 IBA. Adenine sulphate inhibited the number of explants showing regeneration but increased the number of shoot buds per regenerating explant. Shoots were rooted on a 50% strength medium containing 1mg l−1 NAA. Springer-Verlag, 1992 Black pepper nationallicence Piper nigrum nationallicence Clonal propagation nationallicence Shoot tip culture nationallicence AdSO4 : adenine hemisulphate nationallicence BAP : 6-benzylaminopurine nationallicence IBA : indole-3-butyric acid nationallicence NAA : napthaleneacetic acid nationallicence Philip V. Department of Botany, University of Calicut, 673 635, Kerala, India aut Joseph Dominic Department of Botany, University of Calicut, 673 635, Kerala, India aut Triggs G. School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, L3 3AF, Liverpool, UK aut Dickinson N. School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, L3 3AF, Liverpool, UK aut Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 41-44 0721-7714 12:1<41 1992 12 299 https://doi.org/10.1007/BF00232421 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232421 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Philip V. Department of Botany, University of Calicut, 673 635, Kerala, India aut NATIONALLICENCE 700 1- Joseph Dominic Department of Botany, University of Calicut, 673 635, Kerala, India aut NATIONALLICENCE 700 1- Triggs G. School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, L3 3AF, Liverpool, UK aut NATIONALLICENCE 700 1- Dickinson N. School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, L3 3AF, Liverpool, UK aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 41-44 0721-7714 12:1<41 1992 12 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer