caa a22 4500 46902724X CHVBK 20180323132733.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00232415 doi (NATIONALLICENCE)springer-10.1007/BF00232415 In vitro micropropagation of Acacia nilotica subsp. indica Brenan via cotyledonary nodes [Elektronische Daten] [Anita Dewan, Kanan Nanda, Shrish Gupta] Cotyledonary node explants of Acacia nilotica subspecies indica Brenan, differentiated multiple shoots on Gamborg et al.' s medium (B5, Gamborg et al. 1968) supplemented with cytokinins like N6-benzyladenine, 6-(γ, γ-Dimethylallylamino)-purine, kinetin or zeatin. Of the four, BA supported maximum multiple shoot differentiation; the highest average number of shoots (6.3) per expiant was in 1.5 mg/l. The number of shoots was further enhanced by (i) using nodal explants of in vitro regenerated shoots as microcuttings, and (ii) repeated subculture of the original expiants (stumps) on the same medium after excising the shoots. Thus, over seven hundred shoots could be obtained from a single cotyledonary node explant. Individual shoots, when transferred to 2 mg/l indole-3-acetic acid augmented medium organised healthy roots in 100% cultures. Such test tube grown plantlets have been successfully transferred to soil, where they grow well up to eight weeks. Springer-Verlag, 1992 Acacia nilotica nationallicence in vitro regeneration nationallicence cotyledonary node nationallicence leguminous tree nationallicence 2iP : 6-(γ, γ-Dimethylallylamino)-purine nationallicence AC : activated charcoal nationallicence B5 : Gamborg et al. 's medium with 0.8% agar + 3 % sucrose nationallicence BA,N6 : benzyladenine nationallicence CW : coconut water nationallicence FAA : formalin-aceticacid-alcohol nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence Kn : kinetin nationallicence NAA : α-naphthaleneacetic acid nationallicence NOA : β-naphthoxyacetic acid nationallicence Dewan Anita Department of Botany, University of Delhi, 110007, Delhi, India aut Nanda Kanan Department of Botany, Daulat Ram College, University of Delhi, India aut Gupta Shrish Department of Botany, University of Delhi, 110007, Delhi, India aut Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 18-21 0721-7714 12:1<18 1992 12 299 https://doi.org/10.1007/BF00232415 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232415 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dewan Anita Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Nanda Kanan Department of Botany, Daulat Ram College, University of Delhi, India aut NATIONALLICENCE 700 1- Gupta Shrish Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 18-21 0721-7714 12:1<18 1992 12 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer