caa a22 4500 469027274 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00232412 doi (NATIONALLICENCE)springer-10.1007/BF00232412 Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid [Elektronische Daten] [Yuriko Kurioka, Yoshihito Suzuki, Hiroshi Kamada, Hiroshi Harada] Summary: Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of the rolC gene. Springer-Verlag, 1992 Atropa belladonna nationallicence Flowering nationallicence Ri plasmid nationallicence rolC gene nationallicence Transformation nationallicence native rolC : rolC gene under control of its own promoter nationallicence 35S- rolC : rolC gene under control of a cauliflower mosaic viras 35S promoter nationallicence Kurioka Yuriko Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan aut Suzuki Yoshihito Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut Kamada Hiroshi Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan aut Harada Hiroshi Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan aut Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 1-6 0721-7714 12:1<1 1992 12 299 https://doi.org/10.1007/BF00232412 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232412 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurioka Yuriko Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Suzuki Yoshihito Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Kamada Hiroshi Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Harada Hiroshi Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 1-6 0721-7714 12:1<1 1992 12 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer