caa a22 4500 469027282 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00232420 doi (NATIONALLICENCE)springer-10.1007/BF00232420 Induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon ( Cucumis melo L.) [Elektronische Daten] [Isabelle Debeaujon, Michel Branchard] Summary: A procedure leading to the regeneration of whole plants from protoplasts of melon is described. Protoplasts were isolated from cotyledons and leaves of plants grown in vitro. After 14 days of culture, average viability and division rates were respectively 60% and 30% for the two organs, considering total initial protoplasts plated. The manipulation of the exogenous auxin / cytokinin balance in regeneration media enabled to direct morphogenesis towards somatic embryogenesis (1 mg·l−1 2,4-dichlorophenoxyacetic acid and 0.1 mg·l−1 6-benzylaminopurine) or caulogenesis (0.5 mg·l−1 6-benzylaminopurine and 0.5 mg·l−1 kinetin). Contrary to division ability, regeneration capacity was genotype-dependent under our conditions, but the two organs expressed similar division and regeneration capacities. Maltose was superior to sucrose for the development of caulogenic nodules into buds. Some plants were transplanted to soil, where they appeared to be fertile and produced seeds. Springer-Verlag, 1992 Melon nationallicence Protoplasts nationallicence Somatic embryogenesis nationallicence Caulogenesis nationallicence Maltose nationallicence BAP : 6-benzylaminopurine nationallicence CPW : Cell and Protoplast Washing medium nationallicence KIN : kinetin nationallicence MES : 2-(N-morpholino) ethanesulfonic acid nationallicence MS : Murashige and Skoog (1962) nationallicence NAA : 1 — naphthaleneacetic acid nationallicence PAS : H (staining), Periodic Acid-Schiff / Hematoxylin nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence Debeaujon Isabelle Laboratoire de Génétique Végétale, CNRS-URA 115, Université de Paris-XI, Bât. 360, F-91405, Orsay Cedex, France aut Branchard Michel Laboratoire de Biotechnologie et Amélioration des Végétaux, Faculté des Sciences et Techniques, Université de Bretagne Occidentale, 6 avenue Victor Le Gorgeu, F-29287, Brest Cedex, France aut Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 37-40 0721-7714 12:1<37 1992 12 299 https://doi.org/10.1007/BF00232420 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232420 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Debeaujon Isabelle Laboratoire de Génétique Végétale, CNRS-URA 115, Université de Paris-XI, Bât. 360, F-91405, Orsay Cedex, France aut NATIONALLICENCE 700 1- Branchard Michel Laboratoire de Biotechnologie et Amélioration des Végétaux, Faculté des Sciences et Techniques, Université de Bretagne Occidentale, 6 avenue Victor Le Gorgeu, F-29287, Brest Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 37-40 0721-7714 12:1<37 1992 12 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer