caa a22 4500 469027312 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF00232419 doi (NATIONALLICENCE)springer-10.1007/BF00232419 An enzyme-immunoassay of abscisic acid in potato ( Solanum commersonii ) cultured cells [Elektronische Daten] [Stephen Ryu, Paul Li, Mark Brenner] Estimation of abscisic acid (ABA) content in potato (Solanum commersonii) suspension-cultured cells with an enzyme-immunoassay (EIA) was investigated. In crude extracts of potato cultured cells or even after simple clean-up using C18 cartridge, EIA based on commercial monoclonal antibodies (Idetek, Inc) failed to detect any ABA content. An interference could be removed by partitioning against ethyl acetate after the C18 cartridge so that the EIA yielded an estimate of ABA similar to that determined by high pressure liquid chromatography and gas chromatography-mass spectrometry analysis. These results demonstrate the presence of metabolites in potato cultured cell extract that prevent the binding of ABA to its binding site but not the binding of tracer. Springer-Verlag, 1992 Ryu Stephen Laboratory of Plant Hardiness, Department of Horticultural Science and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, Minnesota, USA aut Li Paul Laboratory of Plant Hardiness, Department of Horticultural Science and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, Minnesota, USA aut Brenner Mark Laboratory of Plant Hardiness, Department of Horticultural Science and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, Minnesota, USA aut Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 34-36 0721-7714 12:1<34 1992 12 299 https://doi.org/10.1007/BF00232419 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232419 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ryu Stephen Laboratory of Plant Hardiness, Department of Horticultural Science and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, Minnesota, USA aut NATIONALLICENCE 700 1- Li Paul Laboratory of Plant Hardiness, Department of Horticultural Science and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, Minnesota, USA aut NATIONALLICENCE 700 1- Brenner Mark Laboratory of Plant Hardiness, Department of Horticultural Science and Plant Biological Sciences Program, University of Minnesota, 55108, St. Paul, Minnesota, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 12/1(1992-12-01), 34-36 0721-7714 12:1<34 1992 12 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer