caa a22 4500 469027339 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00232378 doi (NATIONALLICENCE)springer-10.1007/BF00232378 Induction of division and differentiation of somatic embryos in the leaf epidermis of Gaillardia picta [Elektronische Daten] [K. Pillai, I. Rao, I. Rao, H. Ram] Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulators such as naphthaleneacetic acid or indolebutyric acid and benzylaminopurine or kinetin. Within 12 h of culture the epidermal cells showed receding of cytoplasm from the walls. After 48 h of incubation 3 or 4 localized zones, each consisting of 3-8 cells that accumulated cytoplasm and stained densely, were observed. Mitotic divisions occurred in these zones on day 3 of culture and localized masses of callus were observed in 95% of the cultures after 10 days. In another 5 days, the callus differentiated somatic embryos or roots, depending on the growth regulators and their concentration in the medium. Springer-Verlag, 1992 Epidermis nationallicence monolayer culture nationallicence morphogenesis nationallicence somatic embryos nationallicence BAP : 6-benzylamiopurine nationallicence IBA : indolebutyric acid nationallicence Kn : kinetin nationallicence NAA : α-naphthaleneacetic acid nationallicence Pillai K. Department of Botany, University of Delhi, 110007, Delhi, India aut Rao I. Department of Botany, University of Delhi, 110007, Delhi, India aut Ram H. Department of Botany, University of Delhi, 110007, Delhi, India aut Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 599-603 0721-7714 10:12<599 1992 10 299 https://doi.org/10.1007/BF00232378 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232378 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pillai K. Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Rao I. Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Ram H. Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 599-603 0721-7714 10:12<599 1992 10 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer