caa a22 4500 469027347 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00232384 doi (NATIONALLICENCE)springer-10.1007/BF00232384 Protoplast fusion-derived Ogura male sterile cauliflower with cold tolerance [Elektronische Daten] [Thomas Walters, Martha Mutschler, Elizabeth Earle] Cauliflower (Brassica oleracea L. ssp. botrytis) protoplasts with Ogura male sterile and fertile B. oleracea cytoplasms were fused, producing plants with an array of organellar types. Plants with Ogura mitochondria were male sterile; those with B. oleracea chloroplasts were cold tolerant. In some fusions, unfused parental protoplasts were eliminated by double inactivation with iodoacetate and gamma-irradiation; in others, fused protoplasts were physically isolated by micromanipulation or by cell sorting. Double inactivation fusions produced the most plants, including many which were male sterile, female fertile, cold tolerant and diploid. Springer-Verlag, 1992 Brassica oleracea nationallicence Cytoplasmic male sterility nationallicence Organelle segregation nationallicence Selection of fusion products nationallicence IA : iodoacetate nationallicence FDA : fluorescein diacetate nationallicence CMS : cytoplasmic male sterility nationallicence mtDNA : mitochondrial DNA nationallicence Walters Thomas Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA aut Mutschler Martha Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA aut Earle Elizabeth Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA aut Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 624-628 0721-7714 10:12<624 1992 10 299 https://doi.org/10.1007/BF00232384 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232384 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walters Thomas Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Mutschler Martha Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Earle Elizabeth Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 624-628 0721-7714 10:12<624 1992 10 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer