caa a22 4500 469027401 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00232382 doi (NATIONALLICENCE)springer-10.1007/BF00232382 Enhancement of shoot regeneration potential by liquid medium culture from mature cotyledons of sunflower ( Helianthus annuus L.) [Elektronische Daten] [Khalid Chraibi B, Jean-Claude Castelle, Alain Latche, Jean-Paul Roustan, Jean Fallot] We describe here a liquid culture system for the regeneration of shoots at high frequencies from mature cotyledon tissues of three genotypes of sunflower (Helianthus annuus L.) one of which had previously been found to be recalcitrant to regeneration when cotyledons were cultured on solid medium. Cotyledons were excised from 2-day-old seedlings and incubated in liquid Murashige and Skoog's modified medium supplemented with 5.4 μM naphthaleneacetic acid (NAA) and 4.4 μM benzylaminopurine (BAP). After two weeks in culture, the whole upper surface of regenerating explants was covered with green shootlets. The percentages of regenerating explants of three genotypes varied between 60 and 70%, and the number of shoots per regenerating explant was highly increased. The shootlets were transferred to solid Murashige and Skoog's medium allowing shoot development, then to rooting medium. Rooted plantlets were successfully acclimatized and gave fertile plants. The role of liquid medium culture in the induction of sunflower regeneration is discussed. Springer-Verlag, 1992 Chraibi B Khalid Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut Castelle Jean-Claude Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut Latche Alain Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut Roustan Jean-Paul Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut Fallot Jean Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 617-620 0721-7714 10:12<617 1992 10 299 https://doi.org/10.1007/BF00232382 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232382 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chraibi B Khalid Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut NATIONALLICENCE 700 1- Castelle Jean-Claude Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut NATIONALLICENCE 700 1- Latche Alain Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut NATIONALLICENCE 700 1- Roustan Jean-Paul Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut NATIONALLICENCE 700 1- Fallot Jean Laboratoire de Biotechnologie Amélioration des Plantes Ecole Nationale Supérieure Argonomique, 145, avenue de Muret, 31076, Toulouse Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 617-620 0721-7714 10:12<617 1992 10 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer