caa a22 4500 46902741X CHVBK 20180323132733.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00232377 doi (NATIONALLICENCE)springer-10.1007/BF00232377 Isolation of protoplasts from developing barley endosperm: a tool for transient expression studies [Elektronische Daten] [Isabel Diaz, Pilar Carbonero] We have developed a method for the routine isolation of protoplasts from developing starchy endosperm of barley (Hordeum vulgare L.). Preplasmolysis of the intact endosperms, a low concentration of hydrolytic enzymes and gravity sedimentation before any centrifugation step, were crucial factors for a good preparation. Best yields were obtained early after pollination (8-13 days) or with mutants with low starch content. Transient expression of a reporter gene under the control of the 35S promoter, after polyethyleneglycol transfection of endosperm protoplasts, was of the same order as that found in coleoptile derived protoplasts. No significant difference in expression was found for a given tissue between cv. Bomi and its mutant Risø 1508. Springer-Verlag, 1992 Diaz Isabel Centro Nacional de Biotecnologia, CSIC, Madrid, Spain aut Carbonero Pilar Bioquímica y Biología Molecular, E.T.S.Ing. Agrónomos, Universidad Politécnica de Madrid, 28040, Madrid, Spain aut Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 595-598 0721-7714 10:12<595 1992 10 299 https://doi.org/10.1007/BF00232377 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232377 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Diaz Isabel Centro Nacional de Biotecnologia, CSIC, Madrid, Spain aut NATIONALLICENCE 700 1- Carbonero Pilar Bioquímica y Biología Molecular, E.T.S.Ing. Agrónomos, Universidad Politécnica de Madrid, 28040, Madrid, Spain aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 595-598 0721-7714 10:12<595 1992 10 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer