caa a22 4500 469027428 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00232385 doi (NATIONALLICENCE)springer-10.1007/BF00232385 Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato ( Lycopersicon esculentum ) and Lycopersicon chilense [Elektronische Daten] [Augusta Bonnema, Mary O'Connell] Summary: Somatic hybrid plants were recovered following fusion of leaf mesophyll protoplasts isolated from tomato (Lycopersicon esculentum) cultivar UC82 with protoplasts isolated from suspension cultured cells of L. chilense, LA 1959. Iodoacetate was used to select against the growth of unfused tomato protoplasts. Two somatic hybrids were recovered in a population of 16 regenerants. No tomato regenerants were recovered; all of the non-hybrid regenerants were L. chilense. The L. chilense protoplast regenerants were tetraploid. The hybrid nature of the plants was verified using species-specific restriction fragment length polymorphisms for the nuclear, chloroplast and mitochondrial genomes. The somatic hybrids had inherited the chloroplast DNA of the tomato parent, and portions of the mitochondrial DNA of the L. chilense parent. The somatic hybrids formed flowers and developed seedless fruit. Springer-Verlag, 1992 Protoplast fusion nationallicence RFLP nationallicence Mitochondrial DNA nationallicence Chloroplast DNA nationallicence Bonnema Augusta Department of Agronomy and Horticulture, Plant Genetic Engineering Lab, New Mexico State University, 88003, Las Cruces, NM, USA aut O'Connell Mary INRA, Centre de Recherches de Toulouse, LBMRPM, BP27, 31326, Castanet Tolosan, France aut Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 629-632 0721-7714 10:12<629 1992 10 299 https://doi.org/10.1007/BF00232385 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232385 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bonnema Augusta Department of Agronomy and Horticulture, Plant Genetic Engineering Lab, New Mexico State University, 88003, Las Cruces, NM, USA aut NATIONALLICENCE 700 1- O'Connell Mary INRA, Centre de Recherches de Toulouse, LBMRPM, BP27, 31326, Castanet Tolosan, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 629-632 0721-7714 10:12<629 1992 10 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer