caa a22 4500 469027436 CHVBK 20180323132733.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00232380 doi (NATIONALLICENCE)springer-10.1007/BF00232380 A novel type of somatic embryogenesis in Coffea arabica [Elektronische Daten] [Beat Neuenschwander, Thomas Baumann] A novel type of somatic embryogenesis characterized by an efficient and highly synchronized embryo formation was observed in embryogenic callus of Coffea arabica initiated on Murashige and Skoog medium containing kinetin (4 mg/l) and 2,4-D (1 mg/l). It occurs in suspension and goes along with the suppression of "High Frequency Somatic Embryo Induction” (HFSE). This is achieved by favoring during cultivation senescence-or necrosis-like processes which apparently do not impair the competence for embryogenesis. Since the resulting embryos germinate at a rate of 94.5 % without the need of a maturation step, we propose the term "Self-Controlled Somatic Embryogenesis” (SCSE). In addition, HFSE was optimized using half-strength liquid medium with 0.1 mg/l kinetin and 0.25 mg/l 2,4-D for proliferation of embryonic tissue, and 2.6 mg/l ABA for maturation of embryos. Yields as well as germination rates of HFSE embryos were markedly lower as compared to SCSE. Springer-Verlag, 1992 Somatic embryogenesis nationallicence plant regeneration nationallicence cell suspension nationallicence coffee nationallicence Coffea arabica nationallicence ABA : abscisic acid nationallicence BAP : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxy-acetic acid nationallicence NAA : 1-naphthaleneacetic acid nationallicence HFSE : high frequency somatic embryo induction nationallicence LFSE : low frequency somatic embryo induction nationallicence SCSE : self-controlled somatic embryogenesis nationallicence Neuenschwander Beat Institute of Plant Biology, University of Zurich, CH-8008, Zurich, Switzerland aut Baumann Thomas Institute of Plant Biology, University of Zurich, CH-8008, Zurich, Switzerland aut Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 608-612 0721-7714 10:12<608 1992 10 299 https://doi.org/10.1007/BF00232380 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232380 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neuenschwander Beat Institute of Plant Biology, University of Zurich, CH-8008, Zurich, Switzerland aut NATIONALLICENCE 700 1- Baumann Thomas Institute of Plant Biology, University of Zurich, CH-8008, Zurich, Switzerland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 10/12(1992-01-01), 608-612 0721-7714 10:12<608 1992 10 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer