caa a22 4500 469027444 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231838 doi (NATIONALLICENCE)springer-10.1007/BF00231838 High frequency adventitious shoot regeneration from immature cotyledons of pea ( Pisum sativum L.) [Elektronische Daten] [Sebahattin Özcan, Mehdi Barghchi, Simon Firek, John Draper] A procedure has been developed which allows high frequency adventitious shoot regeneration from immature cotyledons of pea. Prolific shoot regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l α-naphthaleneacetic acid (NAA). Cotyledon expiants proximal to the embryonic axis had the highest regeneration potential, however, the presence of an embryonic axis inhibited adventitious shoot regeneration. Addition of silver nitrate (AgNO3) to the medium did not promote the number of regenerated shoots but resulted in shoots with well developed tendrils and large stipules which had a reduced rooting capacity. Regenerated shoots rooted readily (80-90%) in half strength MS medium containing 1 mg/l indole-butyric acid (IBA) and further established well in compost. Springer-Verlag, 1992 Özcan Sebahattin Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut Barghchi Mehdi Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut Firek Simon Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut Draper John Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 44-47 0721-7714 11:1<44 1992 11 299 https://doi.org/10.1007/BF00231838 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231838 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Özcan Sebahattin Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut NATIONALLICENCE 700 1- Barghchi Mehdi Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut NATIONALLICENCE 700 1- Firek Simon Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut NATIONALLICENCE 700 1- Draper John Department of Botany, University of Leicester, LE1 7RH, Leicester, UK aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 44-47 0721-7714 11:1<44 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer