caa a22 4500 469027460 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231839 doi (NATIONALLICENCE)springer-10.1007/BF00231839 Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants [Elektronische Daten] [Attila Fehér, Konstantin Skryabin, Ervin Balázs, Johanna Preiszner, Olga Shulga, Vladimir Zakharyev, Dénes Dudits] Tuber discs of potato (Solanum tuberosum L.) cultivars Desirée and Gracia were infected by Agrobacterium tumefaciens carrying a binary vector with the coat protein gene of potato virus X controlled by the carrot extensin gene long-transcript promoter. Several transgenic potato plants have been obtained by direct regeneration of shoots on culture medium with kanamycin used for selection. The presence of the coat protein gene was proved by Southern hybridization in several transformants. Its low but detectable expression level was shown by Northern and Western analysis. Ethephon treatment resulted in a five-fold increase in the amounts of the coat protein mRNA. The majority of transformants exhibited reduced accumulation of virus RNA in inoculated leaves. Potentials in the use of an ethylene-inducible promoter in the production of virus-resistant transgenic plants will be discussed. Springer-Verlag, 1992 Fehér Attila Institute of Plant Physiology, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB. 521, Hungary aut Skryabin Konstantin Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117 984, Moscow, Russia aut Balázs Ervin Agricultural Biotechnology Center, H-2101, POB. 170, Gödöllő, Hungary aut Preiszner Johanna Institute of Plant Physiology, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB. 521, Hungary aut Shulga Olga Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117 984, Moscow, Russia aut Zakharyev Vladimir Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117 984, Moscow, Russia aut Dudits Dénes Institute of Plant Physiology, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB. 521, Hungary aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 48-52 0721-7714 11:1<48 1992 11 299 https://doi.org/10.1007/BF00231839 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231839 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fehér Attila Institute of Plant Physiology, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB. 521, Hungary aut NATIONALLICENCE 700 1- Skryabin Konstantin Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117 984, Moscow, Russia aut NATIONALLICENCE 700 1- Balázs Ervin Agricultural Biotechnology Center, H-2101, POB. 170, Gödöllő, Hungary aut NATIONALLICENCE 700 1- Preiszner Johanna Institute of Plant Physiology, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB. 521, Hungary aut NATIONALLICENCE 700 1- Shulga Olga Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117 984, Moscow, Russia aut NATIONALLICENCE 700 1- Zakharyev Vladimir Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117 984, Moscow, Russia aut NATIONALLICENCE 700 1- Dudits Dénes Institute of Plant Physiology, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, POB. 521, Hungary aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 48-52 0721-7714 11:1<48 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer