caa a22 4500 469027479 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231830 doi (NATIONALLICENCE)springer-10.1007/BF00231830 Plant regeneration from callus and protoplast cultures of Helianthus giganteus L [Elektronische Daten] [Sergei Krasnyanski, Zsolt Polgár, Gizella Németh, László Menczel] Summary: Methods of plant regeneration from callus and protoplasts of Helianthus giganteus L. are described. Embryogenic callus was obtained from leaf explants and plants were regenerated from these calli on MS media with different combinations of benzyladenine and naphtaleneacetic acid. Leaf protoplasts isolated from in vitro grown plants formed somatic embryos when cultured in agarose solidified droplets of V-KM medium containing benzyladenine and naphtaleneacetic acid. Embryos developed into plantlets on media with reduced auxin contents. Regenerated plants were successfully planted in soil. Springer-Verlag, 1992 Helianthus giganteus nationallicence Protoplasts nationallicence Plant regeneration nationallicence BA : benzyladenine nationallicence IAA : indoleacetic acid nationallicence MS : Murashige and Skoog medium nationallicence NAA : naphtaleneacetic acid nationallicence V-KM : protoplast culture medium of Binding and Nehls nationallicence Krasnyanski Sergei Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary aut Polgár Zsolt Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary aut Németh Gizella Cereal Research Institute, POB 391, H-6701, Szeged, Hungary aut Menczel László Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 7-10 0721-7714 11:1<7 1992 11 299 https://doi.org/10.1007/BF00231830 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231830 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krasnyanski Sergei Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Polgár Zsolt Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Németh Gizella Cereal Research Institute, POB 391, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Menczel László Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 7-10 0721-7714 11:1<7 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer