caa a22 4500 469027509 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231831 doi (NATIONALLICENCE)springer-10.1007/BF00231831 Transformation of Solanum integrifolium poir via Agrobacterium tumefaciens [Elektronische Daten] Plant regeneration and progeny analysis [G. Rotino, D. Perrone, P. Ajmone-Marsan, E. Lupotto] Summary: The wild species Solanum integrifolium represents a source of pest and disease resistance genes for breeding strategies of the cultivated species Solanum melongena. Somatic hybridization via protoplast fusion between the two species may provide a valuable tool for transferring polygenic traits into the cultivated species. The availability of S.integrifolium cells carrying dominant selectable markers would facilitate the heterokaryon rescue. An appropriate methodology for in vitro culture and plant regeneration from leaf explants of S.integrifolium is reported. Efficient leaf-disk transformation via co-cultivation with Agrobacterium tumefaciens led to the regeneration of transformed plants carrying the reporter genes GUS and NPT-II. Transformed individuals were obtained through selection on kanamycin-containing medium. Stable genetic transformation was assessed by histochemical and enzymatic assays for GUS and NPT-II activity, by the ability of leaf disks to initiate callus on Km-containing medium, Southern blot analyses of the regenerated plants, and genetic analysis of their progenies. Selfed-seed progeny of individual transformed plants segregated seedlings capable to root and grow in selective condition, while untransformed progeny did not. Genetic analyses of progeny behaviour showed that the reporter gene NPT-II segregated as single as well as two independent Mendelian factors. In two cases an excess of kanamycin-sensitive seedlings was obtained, not fitting into any genetic hypothesis. Springer-Verlag, 1992 Agrobacterium tumefaciens nationallicence Solanum integrifolium nationallicence transformation nationallicence MS : Murashige and Skoog (1962) medium nationallicence NOS : nopaline synthase nationallicence NPT-II : neomycin phosphotransferase nationallicence GUS : beta-glucuronidase nationallicence LB : Luria and Bertani medium nationallicence KIN : 6-furfurylaminopurine nationallicence BAP : 6-benzylaminopurine nationallicence 2iP : N6-(2-isopentyl)adenine nationallicence ZEA : zeatin nationallicence TDZ : Thidiazuron nationallicence Rotino G. Istituto Sperimentale per l'Orticoltura, P.O. Box 48, 84098, Pontecagnano, SA aut Perrone D. Istituto Sperimentale per l'Orticoltura, Via Paullese 28, 20075, Montanaso L, MI aut Ajmone-Marsan P. Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut Lupotto E. Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 11-15 0721-7714 11:1<11 1992 11 299 https://doi.org/10.1007/BF00231831 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231831 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rotino G. Istituto Sperimentale per l'Orticoltura, P.O. Box 48, 84098, Pontecagnano, SA aut NATIONALLICENCE 700 1- Perrone D. Istituto Sperimentale per l'Orticoltura, Via Paullese 28, 20075, Montanaso L, MI aut NATIONALLICENCE 700 1- Ajmone-Marsan P. Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut NATIONALLICENCE 700 1- Lupotto E. Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 11-15 0721-7714 11:1<11 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer