caa a22 4500 469027517 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231835 doi (NATIONALLICENCE)springer-10.1007/BF00231835 Coat protein mediated resistance to Plum Pox Virus in Nicotiana clevelandii and N. benthamiana [Elektronische Daten] [Ferdinand Regner, Artur da Câmara Machado, Margit da Câmara Machado, Herta Steinkellner, Diethard Mattanovich, Veronika Hanzer, Hans Weiss, Hermann Katinger] Transgenic Nicotiana benthamiana and N. clevelandii plants expressing the coat protein of Plum Pox Virus under the control of the 35S promoter from Cauliflower Mosaic Virus were engineered by Agrobacterium tumefaciens mediated transformation. The phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein and control plants were compared after challenge infection with Plum Pox Virus. N. clevelandii coat protein transgenic plants circumvent virus accumulation. After an initial increase in virus titer similar to the control plants, some coat protein expressing plants showed a reduced accumulation of virus and inhibition of the systemic spread, characterized by decrease of the virus titer and formation of new symptomless leaves. In other N. clevelandii coat protein expressing plants virus accumulation was inhibited and disease symptoms never appeared. N. benthamiana coat protein expressing plants were also protected. After a temporary virus accumulation, virus titer decreased without the appearance of symptoms with the exception of a few plants, which showed a delay of thirty days in the development of symptoms post challenge infection. Springer-Verlag, 1992 Regner Ferdinand University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut da Câmara Machado Artur University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut da Câmara Machado Margit University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut Steinkellner Herta University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut Mattanovich Diethard University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut Hanzer Veronika University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut Weiss Hans University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut Katinger Hermann University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 30-33 0721-7714 11:1<30 1992 11 299 https://doi.org/10.1007/BF00231835 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231835 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Regner Ferdinand University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- da Câmara Machado Artur University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- da Câmara Machado Margit University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Steinkellner Herta University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Mattanovich Diethard University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Hanzer Veronika University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Weiss Hans University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Katinger Hermann University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 30-33 0721-7714 11:1<30 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer