caa a22 4500 469027525 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231833 doi (NATIONALLICENCE)springer-10.1007/BF00231833 Introduction and differential use of various promoters in pollen grains of Nicotiana glutinosa and Lilium longiflorum [Elektronische Daten] [Leonne van der Leede-Plegt, Bernadette van de Ven, Raoul Bino, Theo van der Salm, Arjen van Tunen] Summary: As part of our research to develop an alternative system for the transformation of recalcitrant plant species we investigated the use of the male gametophyte as a transformation vector. Therefore the activity of four different promoters (CaMV 35S, LAT52, chiA PA2 and TR2') was analyzed in pollen of a dicot (Nicotiana glutinosa) and a monocot (Lilium longiflorum) plant species. Gene constructs in which the ß-glucuronidase (GUS) gene was placed under the control of these promoters were introduced in pollen using a particle delivery system. No activity of the Cauliflower Mosaic Virus (CaMV) 35S promoter was detected in pollen of both N. glutinosa and L. longiflorum. The promoter of the tomato flower-specific LAT52 gene was highly active in N. glutinosa pollen but remained silent in L. longiflorum pollen. A similar expression pattern was observed for the pollen-specific Chalcone Flavanone Isomerase chiA PA2 promoter originally isolated from petunia. The TR2′ mannopine synthase promoter of Agrobacterium tumefaciens, however, was active in pollen from Solanaceous species and also in pollen from the monocot L. longiflorum. This suggests that the TR2' promoter is active in vegetative and sporogenous tissues of dicot and monocot plant species. Springer-Verlag, 1992 van der Leede-Plegt Leonne DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut van de Ven Bernadette DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut Bino Raoul DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut van der Salm Theo DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut van Tunen Arjen DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 20-24 0721-7714 11:1<20 1992 11 299 https://doi.org/10.1007/BF00231833 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231833 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van der Leede-Plegt Leonne DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- van de Ven Bernadette DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- Bino Raoul DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- van der Salm Theo DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 700 1- van Tunen Arjen DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700, Wageningen, AA, The Netherlands aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 20-24 0721-7714 11:1<20 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer