caa a22 4500 46902755X CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00231832 doi (NATIONALLICENCE)springer-10.1007/BF00231832 Plant regeneration from protoplasts of wheat ( Triticum aestivum cv. Hartog) [Elektronische Daten] [D. He, Y. Yang, K. Scott] Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium containing half strength of the macroelements, 5 μm 2,4-D and 0.6 M glucose. Colonies were formed at frequencies ranging from 0.1% to 5%. The frequency of colonies forming fully developed plants varied between 1% and 25%. More than eighty green plants with morphologically normal shoots and roots have been obtained and there was no difficulty in establishing these plants in soil. A cytological study of several randomly selected regenerated plants showed the normal chromosome complement for wheat (2n = 42). Springer-Verlag, 1992 Embryogenesis nationallicence Plant regeneration nationallicence Protoplast nationallicence Suspension cultures nationallicence Wheat nationallicence He D. Department of Biochemistry, The University of Queensland, Qld. 4072, Brisbane, Australia aut Yang Y. Department of Biochemistry, The University of Queensland, Qld. 4072, Brisbane, Australia aut Scott K. Department of Biochemistry, The University of Queensland, Qld. 4072, Brisbane, Australia aut Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 16-19 0721-7714 11:1<16 1992 11 299 https://doi.org/10.1007/BF00231832 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00231832 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He D. Department of Biochemistry, The University of Queensland, Qld. 4072, Brisbane, Australia aut NATIONALLICENCE 700 1- Yang Y. Department of Biochemistry, The University of Queensland, Qld. 4072, Brisbane, Australia aut NATIONALLICENCE 700 1- Scott K. Department of Biochemistry, The University of Queensland, Qld. 4072, Brisbane, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/1(1992-02-01), 16-19 0721-7714 11:1<16 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer