caa a22 4500 469027568 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232690 doi (NATIONALLICENCE)springer-10.1007/BF00232690 Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass ( Agrostis palustris Huds.) [Elektronische Daten] [T. Terakawa, T. Sato, M. Koike] A rapidly growing embryogenic suspension culture cell line of creeping bentgrass cv Penncross (Agrostis palustris Huds.) was established from callus derived from the culture of mature seeds. High concentrations of 2,4-D were required for the induction of callus (3 mg/1) as well as for the maintenance of the cell Une (2 mg/1) on modified B5 medium of Gamborg. Protoplasts isolated from the suspension cultures were successfully cultured in Murashige and Skoog's basal medium supplemented with only 0.1 mg/1 2,4-D. Although protoplast plating efficiency was rather low (0.36%), 30% of the protocalli formed normal green plants that were successfully established in soil. Springer-Verlag, 1990 Terakawa T. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan aut Sato T. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan aut Koike M. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 457-461 0721-7714 11:9<457 1992 11 299 https://doi.org/10.1007/BF00232690 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232690 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Terakawa T. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sato T. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan aut NATIONALLICENCE 700 1- Koike M. Central Research Laboratories, Hokko Chemical Industry Co., Ltd., 2165 Toda, Atsugi, 243, Kanagawa, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 457-461 0721-7714 11:9<457 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer