caa a22 4500 469027576 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232691 doi (NATIONALLICENCE)springer-10.1007/BF00232691 Isolation and culture of protoplasts of pigeonpea ( Cajanus cajan L.) [Elektronische Daten] [Bijaya Sarangi, Nickolay Kuchuk, Yuri Gleba] Summary: High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium within three to four weeks of culture. The protocolonies proliferated to form green calli on solid Caboche's medium. No shoots or plants were obtained. Springer-Verlag, 1992 Pigeonpea ( Cajanus cajan ) nationallicence Grain legume nationallicence Protoplast culture nationallicence Callus production nationallicence BAP : 6-benzylaminopurine nationallicence NAA : α-napthaleneacetic acid nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence Kin : kinetin nationallicence Zea : zeatin nationallicence Adn S : adenine sulphate nationallicence GA 3 : gibberellic acid nationallicence Sarangi Bijaya Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Acad. Lebedev Street 1, 252143, Kiev, Ukraine aut Kuchuk Nickolay Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Acad. Lebedev Street 1, 252143, Kiev, Ukraine aut Gleba Yuri Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Acad. Lebedev Street 1, 252143, Kiev, Ukraine aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 462-465 0721-7714 11:9<462 1992 11 299 https://doi.org/10.1007/BF00232691 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232691 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sarangi Bijaya Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Acad. Lebedev Street 1, 252143, Kiev, Ukraine aut NATIONALLICENCE 700 1- Kuchuk Nickolay Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Acad. Lebedev Street 1, 252143, Kiev, Ukraine aut NATIONALLICENCE 700 1- Gleba Yuri Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Acad. Lebedev Street 1, 252143, Kiev, Ukraine aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 462-465 0721-7714 11:9<462 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469027304 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer