caa a22 4500 469027592 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232696 doi (NATIONALLICENCE)springer-10.1007/BF00232696 Transformation of Dendrobium orchid using particle bombardment of protocorms [Elektronische Daten] [Adelheid Kuehnle, Nellie Sugii] Transformed dendrobium orchids (Dendrobium x Jaquelyn Thomas hybrids) were recovered from protocorms bombarded by particles coated with the plasmid pGA482GG/cpPRV4, which contains the plant expressible Nos-NPT II and papaya ringspot virus (PRV) coat protein (CP) genes. Approximately 280 protocorms from four crosses were bombarded and potentially transformed tissues were identified by growth and green color on half-strength Murashige and Skoog medium supplemented with 2% sucrose and 50-100 mg 1−1 kanamycin sulfate. Kanamycin concentrations that prevented growth of nontransformed tissues could not be used for long-term selection because such levels suppressed the regeneration of potentially transformed tissues. PCR and restriction analysis 21 months after treatment found 13 of 13 plants from two crosses, which appeared kanamycin-tolerant, to contain the Nos-NPT II gene, while only one of these plants carried the vector-linked PRV CP-gene. These results support use of particle bombardment for transformation of this important ornamental monocot. Springer-Verlag, 1992 Kuehnle Adelheid Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut Sugii Nellie Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 484-488 0721-7714 11:9<484 1992 11 299 https://doi.org/10.1007/BF00232696 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232696 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuehnle Adelheid Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Sugii Nellie Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 484-488 0721-7714 11:9<484 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer