caa a22 4500 469027614 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232695 doi (NATIONALLICENCE)springer-10.1007/BF00232695 Induction of rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures by yeast extract [Elektronische Daten] [Hajime Mizukami, Terumi Ogawa, Hiromu Ohashi, Brian Ellis] Summary: A transient increase in rosmarinic acid (RA) content in cultured cells of Lithospermum erythrorhizon was observed after addition of yeast extract (YE) to the suspension cultures, reaching a maximum at 24 hr. The highest increase of the RA content (2.5-fold) was obtained when 6-day-old cells in the exponential growth phase were treated with YE. Preceding the induced RA accumulation, phenylalanine ammonia-lyase (PAL) activity increased rapidly, whereas tyrosine aminotransferase (TAT) activity was largely unaffected by the treatment. The incorporation of both 14C-phenylalanine and 14C-tyrosine into RA was enhanced in the YE-treated cells, consistent with increased synthesis of the ester. Springer-Verlag, 1992 Rosmarinic acid nationallicence Biosynthesis nationallicence Lithospermum erythrorhizon nationallicence Induction nationallicence 2,4-D : 2,4 dichlorophenoxyacetic acid nationallicence PAL : phenylalanine ammonia-lyase nationallicence TAT : tyrosine aminotransferase nationallicence RA : rosmarinic acid nationallicence YE : yeast extract nationallicence Mizukami Hajime School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan aut Ogawa Terumi School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan aut Ohashi Hiromu School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan aut Ellis Brian Department of Plant Science, University of British Columbia, 2357 Main Mall, V6T 1Z4, Vancouver, B.C., Canada aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 480-483 0721-7714 11:9<480 1992 11 299 https://doi.org/10.1007/BF00232695 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232695 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizukami Hajime School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan aut NATIONALLICENCE 700 1- Ogawa Terumi School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan aut NATIONALLICENCE 700 1- Ohashi Hiromu School of Pharmaceutical. Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852, Nagasaki, Japan aut NATIONALLICENCE 700 1- Ellis Brian Department of Plant Science, University of British Columbia, 2357 Main Mall, V6T 1Z4, Vancouver, B.C., Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 480-483 0721-7714 11:9<480 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer