caa a22 4500 469027622 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232694 doi (NATIONALLICENCE)springer-10.1007/BF00232694 Regeneration of plants from callus cultures of Origanum vulgare L [Elektronische Daten] [Neena Kumari, P. Saradhi] Investigations were undertaken to achieve rapid multiplication and improvement of Origanum vulgare (a herbaceous, ornamental plant well known for its aromatic and medicinal value) through plant regeneration from callus. The explants (cotyledons, hypocotyl and root segments) excised from 15 d old aseptic seedlings were cultured on Gamborg's B5 medium supplemented with 2,4-D, NAA and BAP individually and in various combinations (at concentrations of 0,10−7,10−6 and 10−5 M). Best callus induction was noted on medium with 10−7 M 2,4-D alone. The cotyledonary expiants proved to be the best source for compact and nodulated callus. The subcultured cotyledonary calli showed shoot induction when transferred onto media supplemented with BAP alone orin combination with 10−7M or 10−6MNAA. However, 10−5M NAA completely suppressed the shoot inducing ability of BAP. In general, NAA promoted root induction from all explants used including cotyledonary callus. Best shoot induction was obtained on medium supplemented with 10−6M BAP+10−6MNAA. Both IBA and NAA at 10−6 M proved to be equally effective in induction of roots from the cut ends of 15-20 mm long shoots (excised from callus) in half-strength B5 liquid medium. Rooted shoots were successfully re-established in soil under controlled conditions. Springer-Verlag, 1992 Kumari Neena Centre of Bioscience, Jamia Millia Islamia, 110025, New Delhi, India aut Saradhi P. Centre of Bioscience, Jamia Millia Islamia, 110025, New Delhi, India aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 476-479 0721-7714 11:9<476 1992 11 299 https://doi.org/10.1007/BF00232694 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232694 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumari Neena Centre of Bioscience, Jamia Millia Islamia, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Saradhi P. Centre of Bioscience, Jamia Millia Islamia, 110025, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 476-479 0721-7714 11:9<476 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer