caa a22 4500 469027630 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232686 doi (NATIONALLICENCE)springer-10.1007/BF00232686 Somatic embryogenesis and plant regeneration in Anthurium andraeanum hybrids [Elektronische Daten] [Adelheid Kuehnle, Fure-Chyi Chen, Nellie Sugii] Summary: A method for the production of somatic embryos and subsequent plant regeneration for Anthurium andraeanum Linden ex André (Monocotyledonae) hybrids is described. Whole leaf blade explants, derived from plantlets grown in vitro, formed translucent embryogénic calli at their basal ends within one month of culture in the dark. Secondary somatic embryos formed frequently and without an intervening callus on surfaces of primary embryos. Embryogenesis was induced with three genotypes using a modified half-strength Murashige and Skoog (MS) medium supplemented with 1.0 to 4.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.33 to 1.0 mg l−1 kinetin. A combination of 2% sucrose with 1% glucose in the medium favored embryogenesis over 3% sucrose alone. Whole leaf blades on medium solidified with 0.18% Gelrite produced more somatic embryos than leaves on medium with 0.7% Bacto-agar. Within two to three months after culture initiation, embryos were transferred to modified MS medium containing 0.2 mg l−1 6-benzyladenine (BA) and 2% sucrose and placed in the light for conversion into plantlets. Rooted plantlets were recovered and transferred into pots with tree fern fiber medium and grown in the greenhouse. Springer-Verlag, 1992 Kuehnle Adelheid Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut Chen Fure-Chyi Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut Sugii Nellie Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 438-442 0721-7714 11:9<438 1992 11 299 https://doi.org/10.1007/BF00232686 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232686 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuehnle Adelheid Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Chen Fure-Chyi Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Sugii Nellie Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 438-442 0721-7714 11:9<438 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer