caa a22 4500 469027649 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232688 doi (NATIONALLICENCE)springer-10.1007/BF00232688 Cloning of Maytenus emarginata (Willd.) Ding Hou — a tree of the Indian Desert, through tissue culture [Elektronische Daten] [T. Rathore, N. Deora, N. Shekhawat] Summary: An in vitro method for cloning and mass multiplication of Maytenus emarginata, a highly drought resistant tree of the Indian Desert, has been developed. Shoot segments harvested from a "plus” tree (30-year-old) were cultured to produce multiple shoots (10-15 shoots/explant) on MS medium containing 0.1 mgl−1 IAA and 2.5mgl−1 BAP. In vitro produced shoots were cut into segments and cultured on shoot proliferation medium but with only 1.0 mgl−1 of BAP to further multiply the shoots. Isolated individual shoots were cultured on a filter paper bridge in half strength MS liquid medium containing 25 mgl−1 of IBA for 72 h in the dark at 28±20 C for induction of root(s). About 70-80 percent of shoots rooted. The treelets developed were hardened and transferred to pots. Around 20,000 plants can be obtained from a single explant within a period of 6 months. The protocol is highly reproducible and efficient. Springer-Verlag, 1992 Maytenus emarginata nationallicence Cloning nationallicence Tissue culture nationallicence Treelet nationallicence Regeneration nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence NAA : α-naphthalene acetic acid nationallicence NOA : β-naphthoxy acetic acid nationallicence BAP : 6-benzylaminopurine nationallicence Kn : 6-furfurylaminopurine nationallicence B5 : Gamborg et al. (1968) medium nationallicence MS : Murashige and Skoog (1962) medium nationallicence Rathore T. Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut Deora N. Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut Shekhawat N. Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 449-451 0721-7714 11:9<449 1992 11 299 https://doi.org/10.1007/BF00232688 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232688 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rathore T. Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut NATIONALLICENCE 700 1- Deora N. Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut NATIONALLICENCE 700 1- Shekhawat N. Plant Biotechnology Laboratory, Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 449-451 0721-7714 11:9<449 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer