caa a22 4500 469027657 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232689 doi (NATIONALLICENCE)springer-10.1007/BF00232689 Endophytic bacteria expressing β-glucuronidase cause false positives in transformation of Dioscorea species [Elektronische Daten] [Mahmut Tör, Sinclair Mantell, Charles Ainsworth] Summary: False positive transformants obtained during plant transformation experiments on species of the monocotyledonous genus Dioscorea (yam) are described. The false positive results were found to be due to endophytic bacteria which exist within aseptically micropropagated shoot cultures and which express β-glucuronidase (GUS). The bacteria were isolated and identified as two species of Curtobacterium. The expression of GUS in these organisms was found to be induced by a variety of glucuronide substrates. The induction of GUS activity in the bacteria can be inhibited by chloramphenicol, tetracycline, ticarcillin and sodium azide. Implications of these results for use of the gus gene in plant transformation work are discussed. Springer-Verlag, 1992 β-Glucuronidase nationallicence Dioscorea nationallicence endophytic bacteria nationallicence false positive transformants nationallicence DTT : Dithiothreitol nationallicence EDTA : Ethylenediamine tetra-acetic acid nationallicence ELISA : Enzyme-linked immuno-sorbent assay nationallicence GUS : β-glucuronidase nationallicence LB : Luria Bertani nationallicence MUG : 4-Methylumbelliferyl-β-D-glucuronide nationallicence PNPG : p-nitrophenyl-β-D-glucuronide nationallicence PVP : Polyvinylpyrrolidone nationallicence SDS : Sodium dodecyl sulphate nationallicence TAE : Tris-acetate-EDTA buffer nationallicence X-Gluc : 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide nationallicence Tör Mahmut Wye College, University of London, TN25 5AH, Wye, Ashford, Kent, England aut Mantell Sinclair Wye College, University of London, TN25 5AH, Wye, Ashford, Kent, England aut Ainsworth Charles Wye College, University of London, TN25 5AH, Wye, Ashford, Kent, England aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 452-456 0721-7714 11:9<452 1992 11 299 https://doi.org/10.1007/BF00232689 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232689 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tör Mahmut Wye College, University of London, TN25 5AH, Wye, Ashford, Kent, England aut NATIONALLICENCE 700 1- Mantell Sinclair Wye College, University of London, TN25 5AH, Wye, Ashford, Kent, England aut NATIONALLICENCE 700 1- Ainsworth Charles Wye College, University of London, TN25 5AH, Wye, Ashford, Kent, England aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 452-456 0721-7714 11:9<452 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer