caa a22 4500 469027665 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232687 doi (NATIONALLICENCE)springer-10.1007/BF00232687 Adventitious shoot induction and somatic embryogenesis with intact seedlings of several hybrid seed geranium ( Pelargonium x hortorum Bailey) varieties [Elektronische Daten] [Javed Qureshi, Praveen Saxena] Summary: Intact seedlings of hybrid seed geranium (Pelargonium x hortorum Bailey) were tested for their ability to produce adventitious shoots and somatic embryos by direct culture of mature seeds on Murashige and Skoog (1962) medium (MS) supplemented with growth regulators BAP, BAP + IAA, or thidiazuron (TDZ). Ten varieties were tested in the presence of different BAP concentrations, four with BAP + IAA, and two with TDZ. Varieties used in this study differed in their response to BAP in the medium. Multiple adventitious shoots were produced by seven of the ten varieties tested. Multiple adventitious shoots were induced at all levels of TDZ in the medium. TDZ also induced callusing from roots and direct embryogenesis from intact hypocotyls. Adventitious shoots were separated, rooted and transferred to soil where they grew as normal healthy plants and flowered. Springer-Verlag, 1992 Geranium nationallicence Pelargonium nationallicence Micropropagation nationallicence Adventitious shoots nationallicence Somatic embryogenesis nationallicence BAP : N6-benzylaminopurine nationallicence IAA : indole-3-acetic acid nationallicence MS : Murashige and Skoog (1962) nationallicence TDZ : thidiazuron nationallicence Qureshi Javed Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Saxena Praveen Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 443-448 0721-7714 11:9<443 1992 11 299 https://doi.org/10.1007/BF00232687 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232687 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qureshi Javed Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Saxena Praveen Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 443-448 0721-7714 11:9<443 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer