caa a22 4500 469027673 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00232685 doi (NATIONALLICENCE)springer-10.1007/BF00232685 Cryopreservation of in vitro-cultured multiple bud clusters of asparagus ( Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification [Elektronische Daten] [H. Kohmura, A. Sakai, S. Chokyu, T. Yakuwa] Summary: A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated "bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to −196°C. Segments were treated with a vitrification solution (PVS2) at 25°C for 45 min or at 0°C for 120 min prior to a direct plunge into liquid nitrogen. After rapid warming, the segments were expelled into Murashige and Skoog medium containing 1.2 M sucrose for 10 min and then plated on agar shoot outgrowth medium. The average rate of shoot formation of vitrified segments producing normal shoots was near 90% without any preculture and/or cold-acclimation treatment. Revived segments resumed growth within 3 days and developed about three shoots per segment. In vitro-cultured bud clusters appear promising as material for cryopreserving asparagus germplasm. Springer-Verlag, 1992 Cryopreservation nationallicence Vitrification nationallicence Asparagus nationallicence In vitro nationallicence cultured bud clusters nationallicence DMSO : dimethyl sulfoxide nationallicence PVS : 2-vitrification solution nationallicence LN : liquid nitrogen nationallicence IBA : 3-indolbutyric acid nationallicence BA : 6-benzylaminopurine nationallicence FDA : fluorescein diacetate nationallicence DSC : differential scanning calorimeter nationallicence Kohmura H. Institute of Biotechnology, Hiroshima Prefectural Agricultural Research Center, Hara, Hachihonmatsu, 739-01, Higashi-hiroshima, Japan aut Sakai A. Asabucho, 1-5-23, Kitaku, 001, Sapporo, Japan aut Chokyu S. Institute of Biotechnology, Hiroshima Prefectural Agricultural Research Center, Hara, Hachihonmatsu, 739-01, Higashi-hiroshima, Japan aut Yakuwa T. Department of Horticulture, Hokkaido University, Kitaku, 060, Sapporo, Japan aut Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 433-437 0721-7714 11:9<433 1992 11 299 https://doi.org/10.1007/BF00232685 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232685 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kohmura H. Institute of Biotechnology, Hiroshima Prefectural Agricultural Research Center, Hara, Hachihonmatsu, 739-01, Higashi-hiroshima, Japan aut NATIONALLICENCE 700 1- Sakai A. Asabucho, 1-5-23, Kitaku, 001, Sapporo, Japan aut NATIONALLICENCE 700 1- Chokyu S. Institute of Biotechnology, Hiroshima Prefectural Agricultural Research Center, Hara, Hachihonmatsu, 739-01, Higashi-hiroshima, Japan aut NATIONALLICENCE 700 1- Yakuwa T. Department of Horticulture, Hokkaido University, Kitaku, 060, Sapporo, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/9(1992-08-01), 433-437 0721-7714 11:9<433 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer