caa a22 4500 469027681 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233368 doi (NATIONALLICENCE)springer-10.1007/BF00233368 Influence of growth regulators on somatic embryogenesis, plantlet regeneration, and post-transplant survival of Echinochloa frumentacea [Elektronische Daten] [A. Sankhla, Tim Davis, D. Sankhla, N. Sankhla, A. Upadhyaya, S. Joshi] After placement on Murashige and Skoog's basal medium supplemented with 3-5 mg/l 2,4-D, immature inflorescence expiants of Echinochloa frumentacea gave rise to three distinct types of callus: a) loosely arranged and soft; b) compact and translucent; c) compact, sticky and mucilaginous. Somatic embryo formation occurred in type ‘b' callus in about 18-24 d. Callus types ‘a' and ‘c' did not produce somatic embryos. The highest percentage of cultures exhibiting somatic embryogenesis occurred on the medium containing 5 mg/l 2,4-D and 0.5 mg/l kinetin. Somatic embryos also formed directly on the inflorescence (without intervening callus formation) in about 15% of the expiants placed on this medium. The addition of paclobutrazol or uniconazole (0.25 or 1 mg/l) to the medium had no influence on the percentage of cultures exhibiting direct somatic embryogenesis, but paclobutrazol slightly increased the mean number of somatic embryos per culture. Many of the callus-derived somatic embryos germinated when subcultured on basal MS medium supplemented with kinetin. Addition of paclobutrazol or uniconazole to the culture medium at 0.25 or 1 mg/l decreased somatic embryo germination and shoot elongation but increased root length and leaf width. Both paclobutrazol and uniconazole increased survival of the plantlets following transplanting to soil. Increased post-transplant survival was accompanied by reduced water loss from plantlets produced on culture media containing triazoles. Springer-Verlag, 1992 Sankhla A. Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut Davis Tim Texas Agricultural Experiment Station, Texas A&M University Research and Extension Center, 17360 Coit Rd., 75252, Texas, Dallas, USA aut Sankhla D. Texas Agricultural Experiment Station, Texas A&M University Research and Extension Center, 17360 Coit Rd., 75252, Texas, Dallas, USA aut Sankhla N. Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut Upadhyaya A. USDA Climate Stress Lab, 20705, Maryland, Beltsville, USA aut Joshi S. Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 368-371 0721-7714 11:7<368 1992 11 299 https://doi.org/10.1007/BF00233368 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233368 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sankhla A. Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut NATIONALLICENCE 700 1- Davis Tim Texas Agricultural Experiment Station, Texas A&M University Research and Extension Center, 17360 Coit Rd., 75252, Texas, Dallas, USA aut NATIONALLICENCE 700 1- Sankhla D. Texas Agricultural Experiment Station, Texas A&M University Research and Extension Center, 17360 Coit Rd., 75252, Texas, Dallas, USA aut NATIONALLICENCE 700 1- Sankhla N. Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut NATIONALLICENCE 700 1- Upadhyaya A. USDA Climate Stress Lab, 20705, Maryland, Beltsville, USA aut NATIONALLICENCE 700 1- Joshi S. Department of Botany, University of Jodhpur, 342001, Jodhpur, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 368-371 0721-7714 11:7<368 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer