caa a22 4500 46902769X CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233361 doi (NATIONALLICENCE)springer-10.1007/BF00233361 High-yield production of valepotriates by hairy root cultures of Valeriana officnalis L. var. sambucifolia Mikan [Elektronische Daten] [François Gränicher, Philippe Christen, Ilias Kapetanidis] Summary: Hairy root cultures of Valeriana officinalis var. sambucifolia were established by infection of sterile plantlets with Agrobacterium rhizogenes strain R1601 The transformed roots were grown in 10 different, hormone-free liquid media and the isovaltrate, valtrate, didrovaltrate, isovaleroxyhydroxydidrovaltrate content was quantified by HPLC. Valepotriates were entirely retained inside the root tissues. The highest overall valepotriate content (10.3 % dry wt), 4 times the amount found in the roots of 9-month-old nontransformed plants, was observed in half strength Gamborg B5 medium supplemented with 2 % sucrose. The hairy roots cultured in Murashige and Skoog liquid medium supplemented with 2 % sucrose for 50 days produced over 44 mg/g dry wt valepotriates. Springer-Verlag, 1992 Gränicher François Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland aut Christen Philippe Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland aut Kapetanidis Ilias Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 339-342 0721-7714 11:7<339 1992 11 299 https://doi.org/10.1007/BF00233361 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233361 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gränicher François Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland aut NATIONALLICENCE 700 1- Christen Philippe Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland aut NATIONALLICENCE 700 1- Kapetanidis Ilias Department of Pharmacognosy, University of Geneva, Sciences II, 30, Quai E.-Ansermet, CH-1211, 4, Geneva, Switzerland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 339-342 0721-7714 11:7<339 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 21416411X BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer